The osmotolerance of Saccharomyces rouxii 48-28 was confirmed with both NaCland KCl-fortified growth media, with more tolerance being exhibited for the potassium salt. Washed and buffered cells from unfortified medium were challenged with a variety of compounds (and also with physical treatments) that potentially would elicit membrane perturbations. The efficacy of these brief treatments was judged primarily by monitoring subsequent viability. Change in the degree of expression of P-fructofuranosidase (EC 3.2.1.26), which is cryptic in young cells of S. rouxii, was a second criterion. There was a linear correlation between cell death and enzyme expression for treatments with polyenes, detergents, some organic solvents which did not denature the enzyme, and various freeze-thaw regimens in graded amounts of glycerol. The species is relatively insensitive to polyene antimycotics, the order of decreasing effect being filipin, nystatin, and amphotericin B. S. rouxii was found to be less sensitive to osmotic shock than is Saccharomyces cerevisiae, but in neither species is P-fructofurano
Hospitals increasingly use a digital platform to market their services as well as reflect the values and mission of the organization. As payment incentives and national initiatives grow for making hospitals more “age friendly” in both the quality and extent of specialized services for older adults, the visibility of these services on a digital platform is of interest. This exploratory, descriptive analysis of hospital websites was to illuminate the ease with which a consumer could identify that a hospital has specialized services in geriatrics as compared with obstetrics. A proportionate stratified random sampling based on the five geographical regions of the country was used to select 220 hospitals with 5% margin of error, 95% confidence interval and 50% sample proportion with no prior information from a population of over 600 hospitals known to have implemented at least one quality initiative specific to older adults (NICHE). A binary matrix was developed for collecting data related to the visibility of services in three main areas of hospital websites: 1) images and terms on the homepage 2) identification of programs in the “Services” function and 3) availability of the specialty in the “Search for Doctor” function. Data show that the frequency of all key words used to identify obstetrical services was greater than those used to describe geriatric services across all functional areas explored in this study. Implications are that hospital websites may be an underutilized resource for marketing services as well as accurately reflecting specialized services for the older adult population.
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