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N ultra-wideline solid-state NMR (SSNMR) spectra were obtained for 16 naturally occurring amino acids and four related derivatives by using the WURST-CPMG (wideband, uniform rate, and smooth truncation Carr-Purcell-Meiboom-Gill) pulse sequence and frequency-stepped techniques. The N quadrupolar parameters were measured for the sp nitrogen moieties (quadrupolar coupling constant, C , values ranged from 0.8 to 1.5 MHz). With the aid of plane-wave DFT calculations of the N electric-field gradient tensor parameters and orientations, the moieties were grouped into three categories according to the values of the quadrupolar asymmetry parameter, η : low (≤0.3), intermediate (0.31-0.7), and high (≥0.71). For RNH moieties, greater variation in N-H bond lengths was observed for systems with intermediate η values than for those with low η values (this variation arose from different intermolecular hydrogen-bonding arrangements). Strategies for increasing the efficiency of N SSNMR spectroscopy experiments were discussed, including the use of sample deuteration, high-power H decoupling, processing strategies, high magnetic fields, and broadband cross-polarization (BRAIN-CP). The temperature-dependent rotations of the NH groups and their influence on N transverse relaxation rates were examined. Finally, N SSNMR spectroscopy was used to differentiate two polymorphs of l-histidine through their quadrupolar parameters and transverse relaxation time constants. The strategies outlined herein permitted the rapid acquisition of directly detected N SSNMR spectra that to date was not matched by other proposed methods.

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