1% Ag Np and 26% ZnO Np were effective against E. faecalis biofilm similarly to conventional endodontic irrigants.
BackgroundIt is unknown whether the bioactivity of Biodentine characterized by the precipitation of hydroxyapatite and formation of tags into the dentinal tubules improve its sealing ability as an apical plug. Aim: To evaluate the sealing ability provided by Biodentine and mineral trioxide aggregate (MTA) apical plugs, with or without phosphate-buffered saline (PBS) intracanal dressing, using a glucose leakage method.Material and MethodsThe space of the canal of 100 root segments with about 12 mm long was shaped using Gates-Glidden. After created an apical retrograde cavity, the root segments were randomly divided into 4 groups (n = 25): G1 – Biodentine; G2 - Biodentine + PBS intracanal dressing; G3 - MTA and G4 - MTA + PBS intracanal dressing. All access openings were filled with temporary cement and all root segments were introduced in floral foams moistened with PBS. After 2 months, all root segments were prepared to evaluate the glucose leakage. The amount of glucose leakage was quantified by a spectrophotometer and the data were analyzed using chi-square test (p<0.05).ResultsTraces of the glucose were observed in a higher of samples that received Biodentine apical plug (p<0.05). The exposure to intracanal PBS did not influence the sealing provided by Biodentine and MTA.ConclusionsThe Biodentine had lower sealing ability than MTA. The interaction with PBS intracanal dressing did not improve the sealing ability provided by sealers. Key words:Apexification, dental cements, dental leakage, glucose, pressure.
RESUMOO objetivo deste estudo foi avaliar a atuação do odontolegista no processo de identificação post mortem do Instituto Médico Legal da cidade de Florianópolis, SC. Para tanto, foi realizada uma análise retrospectiva dos casos de identificação post mortem registrados no Instituto Médico Legal no período de 2005 a 2014. Destes, foram selecionados apenas os laudos periciais de identificação post mortem odontológicos. Foram avaliados 6.511 laudos: os quais 26 foram laudos de identificação post mortem realizados pelo odontolegista. Dentre os 26 laudos, 13 casos foram identificados, sendo que 7 (53,84%) por meio do prontuário odontológico da vítima, 4 (30,76%) por prontuário e exame radiográfico, 1 (7,1%) por prontuário/registro de mordida e placa de acrílico e 1 (7,1%) por foto extrabucal. Os outros 13 casos resultaram em não identificação, pois 12 (46,2%) casos não apresentaram prontuário e em 1 (3,8%) caso houve o incompleto preenchimento do prontuário odontológico, resultando na impossibilidade de identificação dos cadáveres. Conclui-se que a atuação do odontolegista no processo de identificação post mortem no IML Florianópolis forneceu informações eficazes para a identificação de 13 casos em 9 anos e que o método de comparação de prontuário odontológico ante e post mortem foi o mais utilizado nas identificações. DESCRITORES: Antropologia Forense • Odontologia legal • Medicina Legal. ABSTRACTThe purpose of this study is to evaluate the acting of the forensic dentist in the process of the post mortem identification in the National Legal Medicine Institute of Florianópolis, SC. For this matter, a retrospective analysis of post mortem cases identification registered in the National Legal Medicine Institute of Florianópolis, in the period of 2005 to 2014, was done. From these, were selected only the expert reports of odontological post mortem identification. 6.511 expert report were evaluated, though 26 was post mortem identification reports made by the forensic dentist. Among the 26 reports, 13 cases were identified of which 7 (53,84%) through dental records of the victim, 4 (30,76%) mecial record and radiographic examination, 1 (7,1%) record/bite registration material and acrylic plate, and 1 (7,1%) by extraoral photo. The other 13 cases were resulted in no identification, for 12 (46,2%) cases did not present records and in 1 (3,8%) case there was the incomplete filling of the odontological record, resulting in the impossibility of the identification of the corpses. In conclusion, the acting of the forensic dentist in the process of the post mortem identification in the National Legal Medicine Institute of Florianópolis provided effective informations to an identification of 13 cases in 9 years that the method of comparison of the ondontological record ante mortem and post mortem was the most used in the identifications.
Objetivo: O objetivo deste estudo foi avaliar e comparar a efetividade das soluções de hipoclorito de sódio (NaOCl) 1% e 5%, clorexidina (CHX) 2%, suspensões de nanopartículas de prata (Np Ag) 1% e nanopartículas de óxido de zinco (Np ZnO) 26% contra o biofilme de E. faecalis. Material e Métodos: Setenta e seis dentes humanos unirradiculados foram modelados, montados em um aparato específico e esterilizados. Após, 100 μL de uma suspensão de E. faecalis foi inserida nos canais, sendo renovada diariamente por 7 dias. Quatro segmentos radiculares foram analisados por meio de microscopia eletrônica de varredura (MEV) para confirmar a presença do biofilme. Os segmentos radiculares remanescentes foram divididos aleatoriamente em 6 grupos (n = 12), de acordo com a solução irrigadora empregada: G1) solução salina 0,85% (controle); G2) NaOCl 1%; G3) NaOCl 5%; G4) CHX 2%; G5) suspensão de Np Ag 1%; e G6) suspensão de Np ZnO 26%. Concluída a irrigação, a susceptibilidade do biofilme às soluções irrigadoras (n = 10) foi determinada pelo método de plaqueamento e contagem de unidades formadoras de colônias (UFC). Uma análise por meio de MEV foi conduzida em 2 segmentos de cada grupo para visualização da estrutura do biofilme. O conjunto de dados, representados pelos valores médios de UFC para cada grupo, foi analisado estatisticamente pelos testes Kruskal-Wallis e Mann-Whitney (p < 0,05). Resultados: A efetividade das soluções de NaOCl 5% e Np Ag 1% contra o biofilme intracanal de E. faecalis foi superior comparada à solução salina 0,85% (p < 0,05). NaOCl 5% reduziu 100% das UFC comparado ao grupo controle, seguido pela suspensão de Np Ag 1% (97,6%), Np ZnO 26% (96,1%), NaOCl 1% (94,1%) e CHX 2% (93,1%). Conclusão: Com base na metodologia aplicada, as soluções de NaOCl 5% e Np Ag 1% apresentaram excelente efetividade contra o biofilme de E. faecalis estabelecido no canal radicular.Descritores: Biofilmes. Desinfecção. Enterococcus faecalis. Nanopartículas metálicas.
Aim: To analyze the influence of exposure and time of exposure to phosphate buffered saline (PBS) on the push-out bond strength (BS) of Biodentine to dentine. Methods: Eighty 2-mm-thick dentin discs were obtained from transversal sections of human teeth roots. The space of the canal was enlarged using #2-#5 Gates-Glidden burs to obtain 1.3-mm-diameter standardized cavities. The discs were immersed in 17% EDTA followed by 1% NaOCl and dried. The spaces of the canals were filled with Biodentine and the samples were divided into 2 groups (n=40) according to the storage: G1: exposure to moistened cotton pellet; G2: exposure to PBS. After 30 min, 1, 3 and 28 days, 10 samples of each group were subjected to the push-out test. Data were analyzed using ANOVA and Tukey test (p < 0.05). Results: The specimens exposed to PBS had lower BS (p < 0.05), except for the 1-day period (p = 0.6017). In specimens of Group 1 the BS increased up to 3 days (p < 0.05). In specimens in Group 2, the BS increased from 30 min to 1 day (p < 0.0001) and remained stable up to 3 days (p = 0.9876). At 28 days, a significant decrease was observed in the BS values of both groups (p < 0.05). Conclusions: The exposure of Biodentine to PBS led to lower BS values. In general, the BS increased gradually up to 3 days and reduced at 28 days.
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