Nontyphoidal Salmonella (NTS) is a relevant pathogen involved in gastroenteritis outbreaks worldwide. In this study, we determined the capacity to combine the most probable number (MPN) and multiplex polymerase chain reaction (PCR) methods to characterize the most important Salmonella serotypes in raw sewage. A total of 499 isolates were recovered from 27 raw sewage samples and screened using two previously described multiplex PCR methods. From those, 123 isolates were selected based on PCR banding pattern-identical or similar to Salmonella Enteritidis and Salmonella Typhimurium-and submitted to conventional serotyping. Results showed that both PCR assays correctly serotyped Salmonella Enteritidis, however, they presented ambiguous results for Salmonella Typhimurium identification. These data highlight that MPN and multiplex PCR can be useful methods to describe microbial quality in raw sewage and suggest two new PCR patterns for Salmonella Enteritidis identification.
Lincohn Zappelini sem vocês jamais chegaria tão longe, quiçá aqui. À minha avó, Abigail Ferraz de Campos Breternitz (in memorian). 4 AGRADECIMENTOS Como dizia o poeta Vinicius de Morais: "Eu não ando só Só ando em boa companhia" Aos meus pais, obrigada pelo exemplo de verdadeiros lutadores, trabalhadores e íntegros, que me ensinaram tantos predicados valiosos e valores que só se adquirem com uma excelente educação. À minha irmã Brenda Breternitz, que sempre me apoiou em todos os momentos e decisões da minha vida. Ao Lincohn Zappelini, me espelho em você. Obrigada por embrenhar-me nesse mundo da ciência.
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