Citation: Bushra A.M Abdul Azeez, Fatima S. Sebah and Inaam M.N. Alrubayae, Study of Antibacterial and Antifungal Efficacy of Alkaloid Isolated from Nutmeg (Myristica fragrans), J Pure Appl Microbiol., 2019; 13(4):2105-2110. https://doi.org/10.22207/ JPAM.13.4.22 AbstractThe present study included the isolation of alkaloids from the nutmeg fruit (Myristica fragrans) where one of the alkaloid compounds in the nutmeg fruit was found, which is the existence of the aromatic and has carbonyl and phenol groups in addition to nitrogen. The isolated alkaloid showed antibacterial activity against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli), while the alkaloid compound showed an antifungal effect against Candida albicans and Cryptococcous neoformans. No cytotoxicity of human blood solution in concentration (10-200 ppm) was shown in the isolated alkaloid compound. From this result, it was concluded that the alkaloid isolated from Myristica fragrans can be used in treatment of various disorders because of the effectiveness of the compound against bacteria and fungi.