-This study aimed to evaluate phytotoxic effects of glyphosate subdoses in seedlings of rubber tree (Hevea brasiliensis Aubl.). One year after seedlings planting three reduced rates of glyphosate were studied, as follows: T1 -86.4, T2 -172.8 and T3 -345.6 g e a ha -1 and T4 -control (without application). At 7, 14, 21, 28 and 180 days after application (DAA) of herbicide, scores were assigned for visual phytotoxicity, and in the last evaluation were took measurements of increase in height and in trunk diameter plants. Glyphosate drift in H. brasiliensis caused damage to the application at 345.6 g i.a ha -1 dose, reducing plant development in height and diameter, however, there was recovery in the growth of these plants observed after 180 days after herbicide application.
This study evaluated genotoxic and cytotoxic potential of water samples through the analysis of Allium cepa root cells exposed to samples from five urban lakes of Alta Floresta, state of Mato Grosso. The samples were obtained in the dry and rainy periods, October 2014 and April 2015, respectively, at five distinct points. The collection points were as follows: Lake 01, located in MT 206; Lake 02, in Avenida Perimetral Rogério Silva; Lake 03, in Sector C; Lake 04 on Teles Pires Avenue; and Lake 05, in the Cidade Bela District. Bioassays using Allium cepa bulbs were taken from the water at each point, and comparisons were made with the negative and positive controls. The results found the presence of genotoxic and cytotoxic activities on the roots of A. cepa, indicating high potential in cell cycle inhibition. This result may also be caused by the influences of seasonal periods, taking into account that in the rainy season there is a greater transport of cytotoxic substances by rainwater, and in the dry season, a higher concentration of pollutants due to the reduction of water volume, resulting in a greater interaction of the concentrators as well as a greater concentration of the elements found in the water of these lagoons.
Dypsis lutescens is predominant in regions with tropical, subtropical and equatorial climate. Areca, has considerable commercial value in Brazil, is highly cultivated in large scale for landscaping and decoration purposes. The objective of this work was to evaluate the viability of D. lutescens pollen through the staining test, using Lugol 2%, 2% acetic Orcein, 1% Malachite Green, Methylene Blue, 1% Fuchsin, 5% Giemsa and the Reactive of Alexander. For the tested dyes, the viability of the pollen was determined by the staining aspect where it was considered feasible the protoplasm pollen with defined staining and unviable the pollen with stained exina and protoplasm absence. To prepare the slides, the floral buds were cut diagonally with the scalpel to remove the anthers, then the maceration process of the anthers was made with a glass stick, to release the pollen, with a drop of 0.5 mL of each dye, covering the material with a coverslip, after that the slide was observed in an optical microscope at 40x, using the scanning procedure, counting 300 pollen per slide, with 5 repetition for each dye, totalizing 1500 grains. In the present study, it is possible to conclude that Areca D. lutescens palm, although presenting low pollen viability percentages, for some dyes to which it was tested, Acetic orcein 2% Green malachite 1% Methylene blue and Guiemsa 5% showed good results to distinguish viable pollens from unviable ones, being these dyes indicated to test the pollen viability of the species.
The present work aims to estimate the length and width of the seeds, through the analysis of digital images and to validate the methodology through statistical data. To estimate the length and width of seeds via image analysis, 50 M.maripa seeds were used. The seeds were arranged in a decreasing way from 1 to 25 diagonally, on a matte black fabric, on a phenotyping platform, with a Sony Hd Avchd progressive digital Gps camera, coupled at a height of 50 cm. The images were captured by the camera in automatic mode, without flash, automatic ISO speed, in an RGB system and with a size above 2Mb. Soon after the seeds passed the traditional evaluation method with the aid of a digital caliper, measured in terms of length and width. The images were analyzed with the ImageJ software. Statistical analyzes were performed with the aid of the Sigmaplot program. The results of the length and width of the seeds of Inajá seen by the caliper and digital image via camera, were very distant, showing high dispersion and low correlation r = 0.4037 and R² = 0.1629 for length and r = 0.2985 and R² = 0.0891, showing that the Compared data had little similarity. The error between both the methodology was considered low: 3,81063 and 3,769 for the variables of seed length and width. The method of analysis by digital image and caliper for estimating the length and width of Maximiliana maripa seeds showed a low correlation between the two methodologies. The use of image analysis to estimate the length and width of M. maripa seeds is not indicated.
The jambolan as it is popularly known as Syzygium cumini (L.) Skeels, is of Indian origin. Studies in various areas require estimation of leaf area over the growing cycle. Leaf area is an important aspect in the analysis of photosynthetic efficiency of plants. The objective of the present study was to evaluate S. cumini leaf area determination methods based on measurements by caliper, leaf area integrator and previous image calibration in ImageJ Software, comparing both methodologies. The present study was developed at Mato Grosso State University Carlos Alberto Reys Maldonado - UNEMAT, Alta Floresta campus - MT. Fifty leaves were analyzed, which were numbered from 1-50 in length (C) and width (L) with the aid of a digital caliper and leaf area integrator (Leaf area integrator LI-COR 3100), for measuring leaf area (AF), after the sheets were scanned on an HP Photosmart C4680 flatbed scanner and processed using ImageJ software. Given the data found, it was found that the method used via digital imaging was accurate and showed a high correlation with manual measurement, indicating that the morphological characteristics of leaf area, length and width of Jambolão leaves can be estimated through analysis and analysis. Digital image processing.
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