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An in vivo model for perfusion chemotherapy of the rat hind limb is described in which perfusion at a predetermined flow rate of up to 4 ml.min-1 can be rapidly established in a tourniquet-isolated limb. Standard additions to the semisynthetic perfusion medium included glucose, insulin, and lactate from fresh human erythrocytes. Arteriovenous differences in oxygen content revealed a gradual increase with time of the rate of oxygen consumption until a plateau phase was reached. The plateau rates of oxygen consumption, and of utilization of lactate and of acetoacetate, when supplied, were significantly higher at 4 ml flow.min-1 than at 1.2 ml.min-1. When perfusion was performed at 4 ml.min-1, and suitable metabolic substrates were supplied, the rate of oxygen consumption at the plateau level was apparently equal to the estimated in vivo rate.

CH5) and (C7H7+ -CH3), are shifted to higher masses in the spectrum of C7H4D3+ from p-CD3C6H4N02 (Figure 1G), strongly supporting the tolyl structure 3 as the major form of these ions.19Although appearance potentials indicate that C7H7+ ions from m-and p-CH3C6H4Br and o-and m-CHr C6H4I have structure 3 on formation,5 their CA spectra show that only the ions formed from the iodo compounds have retained this structure for 10-5 sec. Similarly, in contrast to previous conclusions,6 our data indicate that ions of structure 3, not 1, are formed from p-c H3C6H4COCH3.(19) However, we find for P-CD3C6H4NO2 and P-CH3C6D4NO2 that the metastable decompositions used in the previous study6 involve complete H/D scrambling, indicating that the differences noted by these authors are due to differences in ion internal energy, not to differences in ion structure.9•16 (20) Postdoctoral fellow supported by grants from the National Institutes of Health and the Army Research Office, Durham.

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