Torque teno virus: Determination of viral genomic loads by genogroup-specific multiplex rt-PCR, detection of frequent multiple infections with genogroups 1 or 2, and establishment of viral full-length sequences. Veterinary Microbiology, Elsevier, 2009, 143 (2-4) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1

It has recently been suggested that a substitution of glutamlne for arginlne at residue 3500 of apolipoprotein (apo) B-100 causes familial defective apo B-100 (FDB), an autosomal, dominantly Inherited disorder, which leads to Increased serum cholesterol levels. From a sample of 243 patients from Munich with type Ma hyperllpoprotelnemia (HL), we have Identified eight individuals with the apo B-100 arglnlne ( j 600) -»glutamine mutation. In a group of 57 subjects with defective low density llpoproteln receptor (LOLR), no mutant apo B alleles were detected. The frequency of FDB In patients with type Ha HL was estimated to be 3%. In the kindreds of three of the probands, 10 additional carriers of the apo B mutation were Identified. Clinical and biochemical data reveal a striking similarity between patients with FDB and those with a defect in the LDLR gene. Our data support previous findings that FDB Is a serious disorder causing premature atherosclerosis.

Summary. The effects of various light intensities on in vivo increases in activities of phosphoriboisomerase, phosphoribulokinase and ribulose-1,5-diP carboxylase and on synthesis of chlorophyll were studied in greening leaves of Hordeutn vullgare L.Each enzyme was already present in dark-grown plants, but further increases in activities required both a light treatiment of the intact plant and a favorable temperature. The amount of enzymatic activity and chlorophyll developed was governed bv light intensity.Measured activities of phosphoriboisomerase and ribulose 1,5-diP carboxylase were highly correlated with synthesis of chlorophyll at all intensities studied. Measured activity of phosphoribulokinase was correlated with synthesis of chlorophyll only at saturating or near saturating light intensities. At decreasing light intensities the response curves of this enzyme differed from those of chlorophyll and of phosphoriboisomerase and ribulose-1,5-diP carboxylase. A lag period of phosphoribulokinase increased with decreasing light intensity. After the lag period a rapid rate of increase occurred which did not level off during 48 hours of illumination. Thus, a different control mechanism may be operative in inducing incrcased activity of this enzyme. (3,9), phosphoribulokinase (9), and ribulose-1,5-diP carboxylase (15,22), increased in activity during greening of barley leaves. Increased activity of phosphori,boisomerase (8) and ribulose-1,5-diP carboxylase (8,13) in greeninig leaves has been demonstrated, but the response of phosphoribulokinase has not been reported. Also, further' investigation was required to determine the response of each of the 3 enzymes in leaves greening at varying light intensity.The relationship of increasing enzymatic activitv to synthesis of chlorophyll in greening leaves is not clear. Several studies indicate a close correlation between the 2. Tromn (20) suggests that fraction 1 protein (23), which contains ribulose-1,5-diP carboxylace (17, 18,20,21), may be part of the protein constituent of protochlorophyll holochrome. This indicates an intimate relationship between synthesis of chlorophyll and that of ribulose-1,5-diP carboxylise. Another report (7) indicates that increased enzymatic activity may be related more to structural development of the chloroplast.The object of the present study was to determine how phosphoriboisomerase, phosphoribulokinase andl ribulose-1,5-diP carboxylase respond in plants treated with varying light intensities and, concurrently, to investigate the relationship of increasing enzymatic activity to synthesis of chlorophyll.

The enantiomeric [1,2-bis(2-hydroxyphenyl)-ethylenediamine]dichloroplatinum(II) complexes were synthesized and their configuration assessed. A preliminary test in the cisplatin-resistant human NIH:OVCAR-3 ovarian cancer cell line, which was previously characterized by its sensitivity against several therapeutically used drugs, showed that both enantiomers produce cytocidal effects in a concentration of 2.5 microM. A difference between the enantiomers became evident from the faster onset of cytocidal activity of the S,S-configurated compound.

SummaryBmkgroimd: The genetic factors that contribute to ischemic heart disease (IHD) are poorly understood, and it is likely that multiple genes acting independently or synergistically contribute to the risk of IHD and outcome. The genes for angiotensin-converting enzyme (ACE) and apolipoprotein E (ApoE) have been implicated independently in the risk oflHD.Hypothesis: This study examined whether genetic polymorphisms in the ACE and ApoE genes are associated with early onset 1HD. Polymorphisms in a third gene, transforming growth factor p2 (TGF p2). with a known role in wound repair and cardiac development, are also examined with respect to early onset ED.Methods: In all, 101 patients with IHD and onset ofdisease before 55 years for men and 60 years for women, and 100 controls with angiographically confirmed normal coronary arteries were recruited for this study. The ACE, ApoE, and TGF p2 genotypes were determined by polymerase chain reaction amplification or Southern blotting and were compared with the patient's clinical and family histories.