Ancient DNA from bones and teeth of 60 individuals from four extinct human populations from Tierra del Fuego-Patagonia (Selknam, Yamana, Kaweskar and Aonikenk) has been extracted and the mitochondrial DNA (mtDNA) amplified by using the polymerase chain reaction. High-resolution analysis of endonuclease restriction site variation in the mtDNA and sequencing of its hypervariable non-coding control region, revealed complete absence of two of the four primary mitochondrial haplotype groups present in contemporary Amerinds, namely A and B. In contrast, haplogroups C and D were found in all but one sample with frequencies of approximately 38% and 60%. These results, together with the decreasing incidence of group A in more southerly latitudes in the American continent and the absence of cluster B above 55 degrees North in America and Asia, argue that the first settlers entering America 21000-14000 years ago already lacked both mtDNA lineages.

Intrapopulational tooth striation variability has been studied in a sample of 99 individuals from the medieval agricultural population of La Olmeda (Palencia, Spain). The number, length, and orientation of all observed striations were recorded using a scanning electron microscope and an image analyzer system. Tooth striations were observed at 100x magnification on the buccal surface of Pm4 and M1 teeth. The results obtained for the adult age group indicate that the buccal striation pattern is a characteristic trait which does not vary significantly among teeth for each individual. Age-group variability suggests that buccal tooth striations accumulate over quite long periods of time. The characteristic striation pattern for the population is completely attained in the subadult age group. For the analyzed population, seasonal changes in dietary habits apparently did not affect the buccal striation pattern. Weaning of children in the population from La Olmeda seems to have occurred long before 2-5 years of age. Infants had a highly abrasive diet, and subadult and adult individuals would have had a slightly softer diet, perhaps due to a higher meat intake. The buccal striation pattern as a dietary indicator seems to be of great reliability, allowing for quantitative analysis of intrapopulation and interpopulation variability.

Buccal microwear has been studied in a sample of 153 molar teeth from different modern hunter-gatherer, pastoralist, and agriculturalist groups, with different diets (Inuit, Fueguians, Bushmen, Australian aborigines, Andamanese, Indians from Vancouver, Veddahs, Tasmanians, Lapps, and Hindus), preserved at museum collections. Molds of an area of the buccal surface have been obtained and observed at 100x magnification in a scanning electron microscope (SEM). The length and orientation of each striation have been determined with a semiautomatic program of an image analyzer system (IBAS). Results show that intergroup variability is significantly higher than the intragroup variability. There exists a tendency toward fewer striations and a higher proportion of vertical striations in the carnivorous groups than in the vegetarian ones. This microwear pattern is concordant with biomechanics (predominantly vertical mandible movements in meat eaters) and phytolith content in plants (more abrasive particles in vegetarian diets). The variability found has been used in a multivariate analysis as a base to compare the microwear pattern of a sample of 20 Middle and Upper Pleistocene fossils, mainly from Europe, analyzed with the same methodology. The sample includes specimens usually classified as archaic H. sapiens (Broken Hill, Banyoles, Montmaurin, La Chaise-Suard, La Chaise-Bourgeios et Delaunay), Neanderthal (La Quina V, Gibraltar 2, Tabun 1 and 2, Amud 1, Malarnaud, St. Cesaire, Marillac), and anatomically modern H. sapiens (Skhül 4, Qafzeh 9, Cro-Magnon 4, Abri-Pataud, Veyrier, La Madelaine, Rond-du-Barry). Results indicate that some of the Neanderthal specimens have a microwear pattern close to that of the carnivorous groups (such as Inuit and Fueguians), suggesting that these individuals follow a hunter strategy. In contrast, archaic H. sapiens and H. sapiens sapiens seem to have a more abrasive diet, probably more depending on vegetable materials, than the Neanderthals.

We have studied the feasibility of using dinucleotide-repeat microsatellites in the analysis of DNA from ancient bones and teeth. We have used three microsatellites (IVS8CA, IVS17BTA, and IVS17BCA) within the cystic fibrosis transmembrane conductance regulator gene in 28 DNA samples from bones and teeth of up to 5000 years of age. PCR amplification was successful in 71.4% of cases. The repeated analysis of each marker produced different genotypes in 97% of samples, and the same individual genotype was reproduced at least once in 45.5% of cases. Alleles differing from the originals consisted of additions or deletions of 1-39 dinucleotides. The mechanism by which alleles differing from the originals were amplified can be related to the marked degradation of the DNA, with repeat sequences of different length interacting with the partially degraded repeats of the amplified loci. The repeated analysis of each sample allowed us to produce data with some anthropological interest. Among the haptotypes detected in samples from Easter Island, two (16-32-13 and 23-32-13) were found in more than one sample. Similarly, three haplotypes (16-7-17, 16-7-13, and 16-24-13) were detected more than once in samples from the Basque Country. Although haplotypes in the Basque Country are amongst the commonest in European chromosomes, most of those detected in the Easter Island samples are not frequent in Europeans. Thus, the repeated typing of microsatellites allowed us to postulate the genotypes that might be present in the samples but dinucleotide markers do not seem to be reliable enough for genotyping ancient bone and teeth samples.

La antropología forense tiene por objeto tanto la identificación del individuo, como la determinación de la causa y circunstancias de la muerte. En este sentido, la antropología forense es esencial para la recuperación de los restos de personas desaparecidas y que fueron enterrados en fosas comunes durante la Guerra Civil y la dictadura franquista, para su posterior retorno a los familiares. En este trabajo se presentan los resultados de la intervención llevada a cabo en la fosa de Gurb, en la que fueron enterrados cuatro soldados republicanos vecinos de Gavà y cuyos familiares habían solicitado su exhumación. El trabajo multidisciplinar llevado a cabo permitió: 1) recuperar toda la información ante mortem disponible en relación a la fosa y los desaparecidos, 2) recuperar mediante metodología arqueológica y directrices antropológico-forenses los restos de los 13 individuos enterrados en la fosa, y 3) analizar los restos en el laboratorio a través de técnicas antropológicas, forenses, moleculares y de superposición craneofacial. Los resultados permitieron tanto la identificación de las cuatro personas buscadas, como la determinación de las causas y circunstancias de su muerte, relacionadas todas ellas con heridas por arma de fuego, acaecidas en un contexto de batalla.