Interleukin-13 (IL-13) is a recently described human lymphokine which is produced by activated T-cells. Its effect on the production of IL-6 by normal keratinocytes and keratinocyte cell lines of human origin was studied and compared to that of IL-4. IL-13, similarly to IL-4, stimulated IL-6 expression by these cells in a dose-and time-dependent manner. Contamination with endotaxin was excluded by the use of polymyxin B and heat-inactivated cytokines. Further, we showed that IL-13, like IL-4, not only stimulated IL-6 production but also was able to induce overexpression of this cytokine in response to an inflammatory signal such as lipopolysaccharide (LPS). In a previous study, we demonstrated that IL-1 3, by inhibiting IL-6 and other cytokines produced by monocytes, exhibited an ~anti-in~ammato~ profile' comparable to that displayed by IL-4, In contrast, we show here that IL-B, by stimulating IL-4 production by keratinocytes, may favour the instaIlation of an ~~~ato~ process at a local level and, here again, it acted like IL-4. Therefore, according to the type of target cell, these two 'TH2 type' cytokines induce similar opposing effects on IL-6 production and are likely to be important cytokines in the regulation of inflation at bath systemic and 1ocaI levels.