In this study the sol-gel method was used to create a composite of titanium oxide nanoparticles (TiO2- NPs) @ ZnO nanoparticles (TiO2 @ ZnO). Using a hydrothermal process, the TiO2@ZnO nanocomposite (NC) was changed via wreathing cadmium sulphide nanoparticles (CdS NPs) (TiO2@ZnO–CdS). Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDAX) were employed to investigate the TiO2@ZnO–CdS NC. The CdS NPs are uniformly distributed over the TiO2@ZnO NC. According to FESEM analysis. TiO2@ZnO–CdS NC is used to detect imidazole at low concentrations and assess cytotoxicity. The TiO2@ZnO–CdS NC has been found to have the best electrochemical sensing activity and considerable cytotoxicity against A549 lung cancer cells.