The gait variables of 10 swimmers, 10 basketball players, and 16 soccer players were compared. They were all male and right-handed. There was no statistical difference between the three groups in age, weight and height. Spatial and temporal gait variables were measured with the Bessou gait analyzer. In the swimmers group, the gait variables of the right side were not statistically different from those of the left side. The right propulsion double support duration, right cycle duration, and right late swing phase duration were respectively longer than those on the left side for the basketball players. The right propulsion double support duration, right step length, and right late swing phase duration were higher than those on the left side for the soccer players. Moreover, a discriminant analysis performed with the gait variables permitted significant differentiation between the three groups. In conclusion, both basketball and soccer players presented asymmetrical gait variables, that have never been previously reported in normal subjects, or in swimmers. These results suggest that the anticipatory postural adjustments programmed to be used just before a jump or a shoot influence the motor program of the spontaneous locomotion. These gait asymmetries could also be due to asymmetric muscle development.