In this work, the seminal parameters of P. mesopotamicus were evaluated fresh and after cryopreservation, focusing on the sperm variables that affect the rates of fertilization, hatching and post-hatching parameters such as larval survival and morphology. The semen and oocytes from the animals were collected after extrusion, and seminal quality and oocyte fertilization were analyzed. Subsequently, a portion of each semen sample was cryopreserved and, after two days, the oocytes from three new females were fertilized with cryopreserved semen from the males. The analyzes showed that progressive motility, spermatic vigor, motility duration, number of normal sperm and secondary abnormalities were higher in fresh semen than in semen after thawing (P <0.0001). Similarly, fertilization and hatching rates and the percentage of normal and abnormal larvae in fertilized oocytes were higher when fresh semen was used (P <0.0001). The cryopreservation process affected the qualitative parameters of the semen of Piaractus mesopotamicus. The primary abnormality of spermatozoa was the main variable that influenced both fertilization and hatching rates, both in fresh and thawed semen. The second most important variable that influenced, particularly, thawed semen, was the spermatic vigor.

Two experiments were carried out to evaluate the effects of rosemary oil (RO) and trace mineral sources (MS) on the internal quality and lipid stability of brown layer eggs. The treatments consisted of diets supplemented with two trace mineral sources (inorganic or organic) and three levels (0, 100, or 200 mg kg

The influence of age (85, 140, and 270 days) of European quails breeders on the egg quality and hatching, fertility and progeny performance was evaluated. The experimental design was completely randomized in a 3x3 factorial arrangement (females' age x males' age), with ten replicates and six birds per experimental unit (four females and two males). Egg production and quality were determined during 3 periods of 14 days and incubation parameters were evaluated in eggs obtained in five consecutive days. The live performance of the progenies was analyzed until 35 days. There was no effect of male age or any interaction between the age of males and females for the evaluated variable. The female's age influenced egg production, egg weight and chick weight, with better results obtained for 140-d-old breeders. The age of females reduced the hatchability, increased the late mortality in incubated eggs, and had no effect on fertility, total embryo mortality or eggshell structure, when analyzed by electron microscopy. The number of sperm trapped in the outer perivitelline layer (sptz/mm²) was determined in 10 fertile eggs per experimental unit. Young females fertilized by young males (80 days) had reduced sptz/mm². Progeny live performance was not affected by breeder's age. Breeders with 270 days retain fertility; however, their egg production, weight and hatchability of fertilized eggs is reduced. In conclusion, European quail breeders with 140 days of age have better egg quality, hatching and breeding results.

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