This study validated a Swedish translation of the Aging Semantic Differential Scale (ASD, 32-items) distributed online. Translation and back-translation were conducted. A convenience sample of nursing students completed the online questionnaire (N = 292) in spring 2020. Confirmatory factor analysis tested a validated four-factor structure consisting of 26 items, and the reliability and validity of the scale were tested. The Swedish version of the ASD was found to be reliable and valid. Model fit indices, internal reliability, and scale validity were acceptable. Construct validity was verified, and mean differences were observed, in accord with previous research regarding participants’ age, sex, clinical experience, and personal relationships with older individuals. The findings provide cross-cultural validation of the ASD by extending its international use. The validation of an online version expands data collection flexibility. As this modified instrument required only 26 items, it may be beneficial for use in future studies and practical settings.