The insulin resistance is especially caused by inflammatory process in obesity. The activation of antiinflammatory cholinergic pathway is responsible to attenuatte inflammation. The objective was investigate if the obese offspring have cholinergic antiinflammatory signalling damage and insulin resistance impaired. The inflammation LPS-induced was able to increase pIKK, IL-6, pSTAT3 levels and decrease gluconeogenesis in lean and obese offspring. The treatment seems to reduce α7nAChR in offspring and increase to pAKT in obese offspring. Our datas indicate which the maternal obesity impaired the cholinergic signalling and this can be affect glucose homeostasis through IL-6 signalling.