Objective: Premnotrypes vorax (P. vorax) is an insect pest that causes significant losses to potato crops in Colombia. Currently, the insect control is mainly done by using highly toxic chemical insecticides and there are no reports of any commercial biological control strategy against this pest. Hence, the objective of this study was to characterize the insect genetic expression to search for genes that could codify for Bacillus thuringiensis Cry toxin receptors. Using an RNA-seq approach, we sequenced the mRNA from the insect tissue, performed a de novo assembly and analyzed the reconstructed transcriptome of P. vorax. To our knowledge, this is the first genetic report of this endemic insect which will set the basis of a possible biological control strategy.Results: The transcriptome data was obtained from dissected midgut tissue samples of P. vorax larvae. The isolated RNA was isolated and sequenced using the Illumina HiSeq platform with a configuration of 2x150pb reads. A total of 383,552,246 reads were obtained and subsequently a quality and cleaning process was performed through FastQC and Trimmomatic software, respectively. A novo assembly was done using the Trinity software, obtaining a transcriptome assembly with 25,631 genes that showed at least one annotation record, resulting in 74,984 transcript isoforms.