Background: Trichophyton is the common genus in dermatophytes fungi in the world. These fungi secreted virulence enzymes that help them take nutrition from the host. Objectives: 1. To determinate enzymes secretion ability of Trichophyton species isolated from patients, including lipase, phospholipase, and protease. 2. To evaluate the correlation between enzyme secretion ability and dermatomycoses clinical forms. Material and methods: Trichophyton species were isolated from the cultivation samples of dermatophytosis patients. Enzyme production experiments were checked by cultivating fungi into suitable solid agar media. Results: The prevalence of Trichophyton spp. producing protease, lipase, and phospholipase were 96.1%%, 86.2%, and 21.6%, respectively. There were 78.4% of fungal strains that produced both lipase and protease, and 17.6% of fungal strains produced three enzymes. The ability to produce protease of T. rubrum was higher than T. mentagrophytes, and there was no statistically significant difference between lipase and phospholipase activities of these two fungal species. There was no difference between the fungal enzymatic activities and dermatophytosis clinical forms. Conclusion: Trichophyton species isolated from patients attending Hue University of Medicine and Pharmacy Hospital produced more protease and lipase than phospholipase. T. rubrum strains showed higher ability protease produce than T. mentagrophytes. Key words: dermatophytes, Trichophyton spp., fungal enzyme

Background: Parasitic and fungal disease is now a public health problem. Objective: To identify the rate of parasite infection at Hue University of Medicine and Pharmacy Hospital from 2015 to 2019. Materials and methods: Retrospective study, survey of laboratory data at the Department of Parasitology from 2015 to 2019. Results: The rates of serum positive for Fasciola sp., Taenia sp., Strongyloides sp., Toxocara sp. was 48.5%, 21.5%, 32.8%, 67.4%, respectively. Infection rates of hookworm, whipworm, strongyloidiasis, small liver fluke, Taenia sp. was 0.8%, 1.7 %, 1.0%, 2.0%, 1.7% respectively. The rate of vaginal yeast, fungal cutaneous infection was 24.2%, 41.1% respectively. Respiratory fungal infection: Candida albicans 31.7%, C. non albicans 21.6%. The rate of fungal infections of the outer ear canal: Aspergillus 54.6%, C. albicans 2.8%, C. non albicans 13%. Conclusion: These results show that the number of parasitic tests is increasing, which corresponds to the importance role of this kind of diseases to the public health. The Parasitology Department play an important role in order for hospital to improve quality to supplying the good service for public health care Key words: protozoa, Parasite, Fungi, Helminth, ELISA

Trichomoniasis is one of the most common sexual transmitted diseases with changable clinical features. The prevalence of metronidazole resistance was different among the studies in the literature. Objectives: The aim of this study was to describe the characteristics of T. vaginalis infection and to investigate the susceptibility with metronidazole of T. vaginalis strains. Materials and methods: A cross-sectional and experimental studies were performed using the interview, the direct examination, the culture of T. vaginalis in Diamond medium, and the investigation of the metronidazole susceptibility by broth microdilution method. Results: The rate of trichomoniasis was 0.37%, in which 16.7% of patients with single status, and 76.7% of those who did not use any contraceptive methods. The common symptoms were increased vaginal discharge, itching and pain (50%); opalescent vaginal discharge (33.3%), The rate of women with asymptomatic T. vaginalis was 26.7%. The percentage of metronidazole susceptibility, intermediate and resistance to metronidazole were 73.1%, 19.2% and 7.7%, respectively. Conclusions: The study showed that T. vaginalis infection had a low rate, high proportion of asymptomatic patients and 7.7% of patients were resistant to metronidazole. Key words: T. vaginalis, metronidazole, resistant

Trichomonosis is the most common non-viral sexually transmitted disease (STD) in the world. Due to the development of metronidazole-resistant isolates, therapeutic alternatives to 5-nitroimidazole are being investigated. T. vaginalis can be naturally infected with Mycoplasma hominis, Mycoplasma girerdii and Trichomonas vaginalis virus (TVV). Objective: 1) The essential oil from Melaleuca quinquenervia was used to test the anti-trichomonas activity. 2) Symbiosis was checked to determine whether there is any correlation between T. vaginalis – infected and T. vaginalis - free with esssential oil. Materials and methods: A total of 32 T. vaginalis isolates from Hue province subjected to susceptibility testing against essential oils by broth microdilution method. Polymerase chain reaction (PCR) was used to detect the presense of M. hominis and M. girerdii. TVV haboring protozoan was identified by total RNA extraction. Result: The M. quinquenervia essential oil showed antitrichomonal activity at the mean of MIC at 0.15 ± 0.07% after 24 hours and 0.09 ± 0.05% after 48 hours. There was no significant differences in MIC of essential oil to T. vaginalis-microorganisminfected and T. vaginalis-free. Conclusion: M. quinquenervia essential oil can be used as potential therapeutic natural resource for development of antitrichomonal drugs, to not only T. vaginalis-free isolates but also T. vaginalis-infected isolates.