In our study of the substances involved in yeast growth stimulation carried out through a period of years, we first investigated extensively the nutrilite requirements of yeast number 578 (2331) of the American Type Culture Collection, and found that several unknown components must be present in order to stimulate its growth1,2'3 one of which may be the antineuritic vitamin4 of Jansen and Donath.Preliminary studies had been made upon a number of yeasts5 and we attempted to find other "strains" of Saccharomyces Cerevisiae which might have simpler requirements. The original strain used by Wildiers in his discovery of "bios" was investigated and the hypothetical "bios" was found beyond question to consist of two substances which were separable by fractional electrolysis, one an acid substance and the other basic.6Our work is now centered upon the requirements of the "Gebriide

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