Electrophoretic method has been applied to biochemical classification of hfugilids in the Lagoons of the Po delta. Among the various proteins analysed the one which turned out to be the most useful was the lactate dehydrogenase enzyme (LDH) because it presents different patterns in the five species living in the above mentioned lagoon and does not show any variations in the course of ontogenesis. This caracteristic makes it possible to identify stocks of fry to be put into closed waters for subsequent cultivation. hloreover this method allows one to make certain phylogenetic speculations with regard to the origin of these species.

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Salamanders collected near Asiago, north of Vicenza (North-eastern Italy), were studied. From their morphological and embryological characteristic and electrophoretic patterns of haemoglobins and LDH isoenzymes they were interpreted as a possible new subspecies of Salamandra a h Law. ACKNO\VLEDGhlENTSWe are grateful to Prof. B. Lanza (Istituto di Anatomia Comparata, Biologia generale e Genetics dell'Universiti di Firenze, Direttore del hluseo zoologico) for the bibliographic advice and the taxonomic confirmation of the materials, and also to hlessrs G. Gnatta and L. Franzan for drawing our attention to the new form.