During storage, red blood cells (RBCs) for transfusion purposes suffer progressive deterioration. Sialylated glycoproteins of the RBC membrane are responsible for a negatively charged surface which creates a repulsive electrical zeta potential. These charges help prevent the interaction between RBCs and other cells, and especially among each RBCs. Reports in the literature have stated that RBCs sialylated glycoproteins can be sensitive to enzymes released by leukocyte degranulation. Thus, the aim of this study was, by using an optical tweezers as a biomedical tool, to measure the zeta potential in standard RBCs units and in leukocyte reduced RBC units (collected in CPD-SAGM) during storage. Optical tweezers is a sensitive tool that uses light for measuring cell biophysical properties which are important for clinical and research purposes. This is the first study to analyze RBCs membrane charges during storage. In addition, we herein also measured the elasticity of RBCs also collected in CPD-SAGM. In conclusion, the zeta potential decreased 42% and cells were 134% less deformable at the end of storage. The zeta potential from leukodepleted units had a similar profile when compared to units stored without leukoreduction, indicating that leukocyte lyses were not responsible for the zeta potential decay. Flow cytometry measurements of reactive oxygen species suggested that this decay is due to membrane oxidative damages. These results show that measurements of zeta potentials provide new insights about RBCs storage lesion for transfusion purposes.

Objectives: The extension project "O Toque pela Vida" was created in 2016 by teachers, administrative technicians and Medicine students from the Federal University of Jataí (UFJ), in partnership with the Jataí's Municipal Secretariat of Health (MSH). It aims to inform and guide the population regarding etiology, risk factors and early detection of Breast Cancer (BC), in addition to providing preventive examinations to women. Methodology: For the project execution, study groups were realized out monthly for the discussion of articles and clinical cases about BC. The information obtained during the groups discussions was used to design pamphlets, banners, posters, T-shirts and ribbons, symbols of the October Pink campaign. Extension activities and educational materials were disseminated on social networks, local radio and television programs. The activities were developed in the main Basic Health Units (BHUs) and in the Raízen Company of Jataí. Among these activities, on October 18, 2017, project students lecture on BC in the Raízen Company. On October 6, 2017, project participants went to BHUs to distribute information materials about BC and to disclose the D-Day Campaign. This campaign took place at BHU James Phillipe Minelli on October 28, and in the external area of the Unit, passwords were given to women for they could receive medical attention and care. Meanwhile, the project members measured the patients' pressure and glycemia. In addition, patients received guidance and clarification about BC, regarding risk factors, symptoms and early detection of cancer. The orientation activities were performed using anatomical breast molds, posters and ribbons distribution. Inside the unit, the patients updated the Unified Health System (UHS) card and filled in the necessary forms for medical care. The medical service were conducted by physicians, along with medical students. During the examinations, population screening for BC was verified, and women over 50 years old and/or presenting changes in clinical examination were scheduled for mammography. Results: During the activities held in the company, around 100 women employees, attended the educational lecture and, at the end, asked questions about BC. The project "O Toque pela Vida" was more widely accepted by the population, compared to the year 2016, which was the beginning of the project. In this year, the actions were focused only on the BC guidelines. However, in 2017, the partnership with MHS and the wide dissemination in the BHUs and media, allowed the project growth. Thereby, the D-Day brought around 200 women to the BHU James Phillipes Minelli. Of the 200 women, 152 were scheduled for mammograms performed by professionals from the Jataí's MHS, 09 were unilateral for diagnosis and 143 were bilateral for tracking the BC. Mammographies were scheduled between October 31 and December 1, 2017. Conclusion: The partnership established between the project "O Toque pela Vida" and the MHS shorted mammography waiting process in the Unified Health System (UHS). Thereby, th...

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