A concentração de creatinoquinase (CK), aspartato aminotransferase (AST) e dehidrogenase lática (LDH) foi determinada em amostras de soro obtidas de 60 equinos da raça Crioula: 20 éguas mantidas no pasto (Grupo A), 20 equinos em treinamento (Grupo B) e 20 participantes da competição do "Freio de Ouro de 1997" em Esteio - RS (Grupo C), dos quais foram colhidas amostras 24 a 48 horas antes do início da competição e 24 e 48 horas após a mesma. Não houve variação significativa na LDH. O grupo B apresentou concentrações de CK e AST mais elevadas (p<0,05) que os grupos A e C, indicando uma adaptação ao exercício. Quarenta e oito horas após a competição, a CK alcançou a sua concentração mais baixa e, a AST, a mais elevada. Isso indica que a CK e AST são mais informativas para avaliação da função muscular que a LDH. As fêmeas apresentaram concentração de CK mais elevada (p<0,05) que os machos. Houve variação significativa com relação à classificação dos equinos em competição, mostrando uma tendência a valores mais baixos nas primeiras colocações, o que indicaria a condição dos equinos melhor preparados.
-The objective of this study was to evaluate the efficacy of cleaning and decontamination procedures in liquid nitrogen tanks. We evaluated 151 canisters and 133 bottoms from 133 nitrogen tanks of companies or farms for the presence of bacteria and fungi. Samples were collected from the canisters and the bottom of tanks containing liquid nitrogen. Tanks were divided into Group 1 (G1): tanks decontaminated with 2% glutaraldehyde -Glutaron ® II (n = 16 canisters in 8 tanks); Group 2 (G2): decontamination with 70% ethanol (n = 20 canisters in 10 tanks); and Group 3 (G3): decontamination with 70% ethanol (n = 115 canisters in 115 tanks). Tanks in Groups 1 and 2 belonged to companies; Group 3 tanks belonged to farms. The culture of canisters showed twelve genera of bacteria and five genera of fungi. Bacillus cereus was the most prevalent bacterial contaminant (42/133) in liquid nitrogen tanks (31.57%). Decontamination by 2% glutaraldehyde plus 70% ethanol was effective and no difference was found between the decontamination methods of Groups 1 and 2. In Group 3 the decontamination method was considered effective. Handling procedures with high hygienic standards should be recommended to avoid contamination of liquid nitrogen tanks on farms.
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