Vibrio harveyi is a marine bacterial pathogen responsible for episodic epidemics generally associated with massive mortalities in many marine organisms, including the European abalone Haliotis tuberculata. The aim of this study was to identify the portal of entry and the dynamics of infection of V. harveyi in the European abalone. The results indicate that the duration of contact between V. harveyi and the European abalone influences the mortality rate and precocity. Immediately after contact, the epithelial and mucosal area situated between the gills and the hypobranchial gland was colonized by V. harveyi. Real-time PCR analyses and culture quantification of a green fluorescent protein-tagged strain of V. harveyi in abalone tissues revealed a high density of bacteria adhering to and then penetrating the whole gill-hypobranchial gland tissue after 1 h of contact. V. harveyi was also detected in the hemolymph of a significant number of European abalones after 3 h of contact. In conclusion, this article shows that a TaqMan real-time PCR assay is a powerful and useful technique for the detection of a marine pathogen such as V. harveyi in mollusk tissue and for the study of its infection dynamics. Thus, we have revealed that the adhesion and then the penetration of V. harveyi in European abalone organs begin in the first hours of contact. We also hypothesize that the portal of entry of V. harveyi in the European abalone is the area situated between the gills and the hypobranchial gland.T he halophilic Gram-negative bacterium Vibrio harveyi is a common pathogen of many marine vertebrate and invertebrate species (1). V. harveyi is known to induce gastroenteritis, inflammation of the circulatory system or eye, and skin lesions in various species of fishes, crustaceans, and mollusks (2). In the European abalone Haliotis tuberculata, V. harveyi is responsible for the massive mortalities that occur during the summer spawning period both in natural populations and in farmed stocks (3, 4). Vibriosis of the European abalone is characterized by the presence of white spots on the foot and inflammation of the pericardial tissue, resulting in impaired mobility and septicemia (5).Schematically, the bacterial infection process can be summarized to occur in three stages: (i) colonization, adhesion of the pathogen to the host surface, and initial multiplication and then penetration of the pathogen into the body through one or several portals, (ii) invasion of the host organs and/or the circulatory system concurrently with the expression of virulence factors by the pathogen, and (iii) exit of the pathogen and transmission of the disease (6, 7). Invasion and multiplication of V. harveyi inside the circulatory system, the hemolymph, seem to take place after 24 h of contact with the European abalone (5), suggesting that the adhesion and penetration of V. harveyi must occur in the first 24 h of contact. The portal of entry and the precise timing of the earlier stages of V. harveyi infection in the European abalone remain uncharacterized....