Objective: The objective of the study was to investigate the cytotoxic activity of hydroalcoholic extract of a polyherbal powder on Vero (normal kidney) and Chang (normal liver) cell lines. Methods: The cytotoxic effect was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide assay. It is commonly used colorimetric assay for assessing cell metabolic activity under specific conditions, indicates the number of viable cells. Results: According to the experimental results, the hydroalcoholic extract does not cause any toxicity on normal liver and kidney cell lines and LC50 values are ca lculated using LDP line software and found 776.73 μg/ml and 686.58 μg/ml. Conclusion: The hydroalcoholic extract of polyherbal churnam exhibited cell viability at higher concentrations. The results indicates investigated formulation does not causes any toxicity to the normal liver and kidney cells and found to be safe for human consumption. Further, toxicological and pharmacological evaluation is required to prove its safety and efficacy.

Diabetes mellitus is a group of metabolic disease in which a person experiences high blood glucose levels either because the body produces inadequate insulin in the body. Though there are several treatment options available there are limitations such as high costs and side effects, weight gain etc. For this reason, the use of medicinal plants has increased to be used as an anti-diabetic agent with less side-effect and more efficient. In this regard, this study analyzed the anti-diabetic potential of Garcinia kola and Syzygium cumini using alpha amylase inhibition assay and glucose uptake by yeast cells. It was observed that Ethanol extract of Garcinia kola increased anti-diabetic potential compared to Syzygium cumini.

Millet mix was prepared for its traditional values. The prepared sample was analyzed for its chemical and nutritional value, and by using the cost-efficient method, the nutritional content of the final product was enhanced. The improved sample was checked for its nutritional content. The objective is to make a comparison between standard and enhanced samples. ingredients were prepared in a powdered form of a sample in four different ratios. The standardized ratio of the ingredients used to make the samples were found by using tests. In the standardized ratio normal (S1) and nutrition enhanced (S2) samples were prepared using the three ingredients. For nutrition enhancement, the method of sprouting was used. Various tests were conducted for the standardized sample to verify its nutritional content, commercializing ability, microbial analysis, analysis etc. The nutritional content Analysis of the normal and nutrition enhanced samples (S1) and (S2) was done. The sample (S2) was nutritionally rich when compared to the normal sample (S1). All other test had more or less coinciding results for both the samples (S1) and (S2). By comparing the nutritional content, a conclusion arrives that the sample (S2is nutritionally rich when compared to (S1). Microbial and Physical properties results show that the product is efficient to be commercialized and stored to a specific period without microbial contamination in powdered form. The nutrient-rich mass is suitable for all age group.

Objectives: The aim of the study was curing antipsoriasis through Euphorbia hirta. The antipsoriasis activity was done by the 3-[4,5-dimethylthiazol- 2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay method.Methods: Aerial parts were shade, dry for 2 days, make into coarse powder and soaked in methanol for 72 h. The supernatant liquid was filtered by Whatman filter paper and condensed in a hot plate at 50°C. Dark gummy mass obtained. The study identified the antioxidant, antimicrobial, and antipsoriasis activities of methanol extract of aerial parts of E. hirta.Results: The IC50 value of methanol extract of aerial parts was found to be 72.20 μg/mL, 97.88 μg/mL, 55.88 μg/mL, and 36.31 μg/mL by 1,1-diphenyl- 2-picrylhydrazyl radical scavenging assay, superoxide radical scavenging assay, phosphomolybdenum reduction assay, and ferric (Fe3+) reducing power assay. The antipsoriasis activity was done by the MTT assay method. The maximum cell death was 88.37% observed at 0.781 μg/mL concentration and IC50 was 12.20 μg/mL concentrated.Conclusion: The results of the present investigation reveal the antipsoriasis activity of the extracts of E. hirta against bacteria and viruses, the psoriasis causing organisms. The methanol extract of E. hirta shows antioxidant, antimicrobial, and antipsoriasis activity and can be used to formulate a potential therapeutic agent for psoriasis.

Objectives: Antioxidant activity was studied in naturally dried seed extract and hot air oven dried extract of Actinidia deliciosa and Psidium guajava using ferric reducing antioxidant power (FRAP) assay method. Methods: The dried powdered seed of A. deliciosa and P. guajava 10 g was dissolved in 100 ml of ethanol in four different conical flasks S1 (for naturaly dried seeds of A. deliciosa), S2 (for hot air oven-dried seeds of A. deliciosa), S3 (for naturally dried seeds of P. guajava), and S4 (for hot air oven-dried seeds of P. guajava). The extract was carried out in shaker at 120 rpm for 72 h at room temperature by mild shaking. The extract was taken out and centrifuged at 4000 rpm for 10 min, the supernatant was taken out. The supernatant was placed in a water bath at 95°C for the solvent to evaporate and stored at room temperature. Results: According to the FRAP results, P. guajava which was naturally dried and extracted has shown the highest antioxidant activity (sample 3) then followed by the samples S4, S1, and S2. The least activity is observed in the sample (S2). Conclusion: By comparing the antioxidant activity between the A. deliciosa and P. guajava with the help of FRAP assay results, P. guajava has the highest amount of vitamin C (responsible for antioxidant activity) when compared to that of the A. deliciosa.