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Chemical separations are an important part of an undergraduate chemistry curriculum. Sophomore students often get experience with liquid−liquid extraction in organic chemistry classes, but liquid−liquid extraction is not as often introduced as a quantitative sample preparation method in honors general chemistry or quantitative analysis classes. This experiment offers a relevant, interesting way to use liquid−liquid extraction and high-performance liquid chromatography (HPLC) to analyze popular lotions and creams for niacinamide content. After completing the experiment, students will have a better understanding of the extraction technique, Beer−Lambert law, and HPLC. The percent recovery from a spiked sample of Olay Original Active Hydrating Beauty Fluid was found to be 99.22% with a standard deviation of 0.01%.