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Data pertaining to the endogenous mouse spleen colony system, 10 days postirradiation, are presented. The Do for visible colonies is 78 R, while that for 6 hr iron uptake over a range of 600–800 R is 50 R. The Do for spleen weight is 196 R and that for IUdR uptake is 193 R. These measurements increase with the age of the mouse. Hypertransfusion decreases spleen iron uptake and colony number. DF-32P and sodium sulfate-35S are not useful indicators of splenic hematopoiesis in this system. Visible hematopoietic colonies in the spleen are not produced by vinblastine, nitrogen mustard, methotrexate, or cyclophosphamide.

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We report the successful management of a 25 year-old woman diagnosed in the second trimester of her pregnancy with chronic myelogenous leukemia (CML). She was treated with leukapheresis until her delivery at 38 weeks of gestation. The procedure was without significant adverse effects on the patient or the fetus. Following induced vaginal delivery, the patient underwent allogeneic bone marrow transplantation from her HLA-matched brother and is presently disease free 13 months following her transplant.

A B S T R A C T A millipore diffusion chamber system was used to cultivate mouse marrow in the abdomens of irradiated and unirradiated host mice for 24 hr. When the irradiated hosts were 72, 96, or 120 hr postirradiation, the number of blasts and promyelocytes in the implanted chambers after cultivation was greater than those in the same marrow cultivated in unirradiated hosts. These data indicate that in vivo, there is stimulation of granulocytopoiesis by a diffusible factor or factors. INTRODUCTIONThe concept that one or more soluble substances may be responsible for the homeostatic control of granulocytopoiesis is supported by recent experimental evidence. The demonstration that hematopoietic colony formation in vitro in agar is enhanced by a factor in mammalian urine or plasma (1-3) suggests that this factor may play a role in the regulation of granulocyte production in vivo. In other studies, DNA synthesis in vitro by granulocyte precursors was enhanced by a plasma factor from leucapheresed animals, as well as retarded by a soluble factor from viable granulocytes (4).The demonstration that such factors control granulocytopoiesis in vivo has been difficult because such a demonstration requires (a) that the marrow cells affected be accurately quantified and (b) that the test marrow be isolated from cell-mediated stimuli.