ABSTRACT. The fatty acid and natural product content of hemp seed oil was analyzed by GC-MS and LC-MS. The presence of linoleic acid (LA) and a-linolenic acid (LNA) were confirmed in their previously reported ratio of 3:1 LA:LNA. The presence of b-caryophyllene (740 mg/L), myrcene (160 mg/L), b-sitosterol (100-148 g/L) and trace amounts of methyl salicylate was observed in the oil which had not been previously reported. Trace amounts of cannabidiol (CBD) were also detected. Bioassays were performed with the oil to determine its effectiveness as an antimicrobial agent. Some bioactivity was observed during the primary screening.