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Antigen preparation from plant tissues of pathogenic mycoplasms (MLO) causing flavescence doree diseaseExtraction and purification of plant yellow's pathogen mycoplastns {MLO) from plant tissues is a difficult problem. It concerns mdeed non cultivable, heterogeneous and fragile organisms which are localized in the fihrous and resistant phloem tissue. In a work directed hy an infectivity test by ieafhopper injection, our lahoratory investigated the best host plant and the best extraction method for this type of pathogen.Broad bean, Vicia fuba gave better extracts than Vitis vmifera. Stems are better than petioles or lamina. The best results were obtained with the top region of the stem, at the level where symptoms are apparent on young plants.The most efficient mincing method is achieved with razorblades moved alternatively by the rapid vertical movement of an electric knife.

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