BACKGROUND AND OBJECTIVES: Inflammation is a defense response of the body to a cellular damage caused by physical, chemical or biological agents, which triggers, among other factors, pain. Although inflammation plays an important role in the protection and regeneration of tissue injury, inflammatory pain results in decreased quality of life. In view of this, the development of safe and less invasive forms for the treatment of inflammatory pain is of great importance. The objective of this study was to evaluate the antihyperalgesic potential of the culture supernatant of keratinocytes and human fibroblasts in an experimental model of inflammatory hyperalgesia. METHODS: Evaluation of carrageenan induced inflammatory hyperalgesia through the use of electronic von Frey in animal models treated with culture supernatant of keratinocytes and fibroblasts. RESULTS: Local administration of naloxone, a nonselective opioid antagonist, in peripheral tissue, has been observed to inhibit the antihyperalgesic effect of the keratinocyte culture supernatant. Fibroblast culture supernatant on days 1 and 3 reverses for 2 hours the carrageenan induced inflammatory hyperalgesia, which is mediated by µ opioid agonist. CONCLUSION: This study indicates that culture supernatant of fibroblasts and keratinocytes is capable of inducing antinociception in inflammatory hyperalgesia, mediated by the release of Evaluation of the keratinocytes or fibroblasts culture supernatant in an inflammatory hyperalgesia model Avaliação do sobrenadante da cultura de queratinócitos ou fibroblastos em modelo de hiperalgesia inflamatória

Objetivos: Estimar a prevalência de pessoas com estomia de eliminação e caracterizá-las quanto aos aspectos sociodemográficos e clínicos. Método: Estudo transversal descritivo com amostra de 27 pacientes com estomias de eliminação, residentes em uma microrregião do norte de Minas Gerais, que atenderam aos critérios de inclusão. Os dados coletados foram registrados em instrumento utilizado em estudos anteriores e analisados por meio de estatística descritiva. Estudo aprovado pelo Comitê de Ética. Resultados: A prevalência de pessoas com estomia foi de 2,16/10.000, a amostra continha 55,6% de homens, 40,7% eram casados, com média de idade de 59,1 anos, 59,2,% eram alfabetizados e 74,0% tinham baixa renda; 59,3% das estomias decorreram de câncer, sendo mais frequentes os de cólon e reto, 70,4% das estomias eram definitivas e 74,0% eram colostomias. Dermatite foi a principal complicação e 68,2% dos pacientes apresentavam efluente de consistência pastosa e realizavam autocuidado. Conclusão: A prevalência de pessoas com estomia foi semelhante à de outros municípios de Minas Gerais e abaixo do esperado pelas Associações Nacional e Internacional de Ostomizados. A caracterização das pessoas, das estomias e do manejo destas contribuirá para que profissionais de saúde e gestores revisem os protocolos de assistência utilizados na microrregião estudada.

This study aimed to raise demographic and epidemiological aspects as well as the time until surgery in patients with brachial plexus injury in Minas Gerais, Brazil. Forty-seven patients who underwent surgery were evaluated retrospectively in the period 2010 to 2011. The majority (91.5%) were male, mean age 34.4 years; 56.5% were single or separated; 55.3%, from the countryside of Minas Gerais. With regard to schooling, 47.8% had not completed elementary school level. Most of them (43.5%) had a heavy duty job, including houseworking, agriculture, industry. Traffic accidents were the main cause of the injury (97.9%); and motorcycle accidents accounted for 68.1% of the lesions. The time between the accident and the first view by brachial plexus specialist ranged from 1 month to 30 months. The time between query and specialized surgical treatment ranged from 5 to 18 months, mean 10 months. This study points out the epidemiological characteristics of patients with brachial plexus injuries in Minas Gerais, Brazil. Most of the injuries occurred after motorcycle accident in male patients with unfavorable social and economic conditions. In addition, the surgical timing was far above the recommended, resulting in an unfavorable prognosis for functional recovery. Palavras-chaveResumo O presente estudo objetivou levantar aspectos sociodemográficos e epidemiológicos, bem como o intervalo de tempo até a cirurgia, dos pacientes com trauma do plexo braquial em Belo Horizonte (MG). Quarenta e sete pacientes submetidos à cirurgia foram avaliados retrospectivamente no período de 2010 a 2011. A maioria (91,5%) era do sexo masculino, com média de idade de 34,4 anos; 56,5% eram solteiros ou separados; 55,3%, provenientes do interior do estado de Minas Gerais. No que se refere ao nível de escolaridade, 47,8% não haviam completado o nível de ensino fundamental. A maioria deles (43,5%) exercia atividade braçal (serviços gerais, agricultura, indústria e

BACKGROUND AND OBJECTIVES: Inflammation is a defense response of the body to a cellular damage caused by physical, chemical or biological agents, which triggers, among other factors, pain. Although inflammation plays an important role in the protection and regeneration of tissue injury, inflammatory pain results in decreased quality of life. In view of this, the development of safe and less invasive forms for the treatment of inflammatory pain is of great importance. The objective of this study was to evaluate the antihyperalgesic potential of the culture supernatant of keratinocytes and human fibroblasts in an experimental model of inflammatory hyperalgesia. METHODS: Evaluation of carrageenan induced inflammatory hyperalgesia through the use of electronic von Frey in animal models treated with culture supernatant of keratinocytes and fibroblasts. RESULTS: Local administration of naloxone, a nonselective opioid antagonist, in peripheral tissue, has been observed to inhibit the antihyperalgesic effect of the keratinocyte culture supernatant. Fibroblast culture supernatant on days 1 and 3 reverses for 2 hours the carrageenan induced inflammatory hyperalgesia, which is mediated by µ opioid agonist. CONCLUSION: This study indicates that culture supernatant of fibroblasts and keratinocytes is capable of inducing antinociception in inflammatory hyperalgesia, mediated by the release of Evaluation of the keratinocytes or fibroblasts culture supernatant in an inflammatory hyperalgesia model Avaliação do sobrenadante da cultura de queratinócitos ou fibroblastos em modelo de hiperalgesia inflamatória

No abstract