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This chapter briefly focuses on the use of biodiesel and natural gas in dual-fuel diesel engines, providing information about natural gas and biodiesel, and their characteristics, engine performance and exhaust gas emissions. The theoretical and experimental study provides analysis of a commercial compression ignition engine operating in dual mode with three fuels: natural gas, diesel and biodiesel at different load and biodiesel blends. On the basis of the results, the effects of air conditions, and the type and quantity of fuel used over the engine performance and environmental impact are reported, and viability of natural gas and biodiesel as alternative fuels for diesel engines is verified.