A new method for the preparation of 3,6-dibromopyridazine makes it possible to employ this substance in the synthesis of 3-sulfanilamido-6methoxypyridazine which has recently shown great promise as a drug. 3,6-Dibromopyridazine is much more reactive than the corresponding dichloropyridazine and may be used to advantage in the following reactions.Phosphorus oxybromide (3 parts) reacted vigorously with 3,6-dioxohexahydropyridazine (1 part) at 70-80°for 2 hours; the OH groups were substi-

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The possible presence of steroids in the tissue of induced hormone-dependent rat mammary tumours was investigated. The method used involves a preliminary extraction of tumours followed by chemical separation and thin-layer chromatography. The identified compounds were cholesterol, androst-4-ene-3,17-dione, 5beta-androst-1-ene-3,17-dione, androsta-1,4-diene-3,17-dione and oestrone. This is the first report of the presence of these steroids in the tissue of an experimental tumour of a non-endocrine organ. In particular 5beta-androst-1-ene-3,17-dione has not previously been identified from natural sources.

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