Penelitian bertujuan untuk mendapatkan isolat bakteri endofit dari akar, daun dan batang Salawaku (Falcataria mollucana) dan menguji kemampuan antagonis bakteri tersebut dalam menekan pertumbuhan cendawan patogen Cercospora spp penyebab penyakit bercak merah pada tanaman Samama (Anthocephalus macrophyllus).Isolasi dilakuka dari dua tanaman sampel yang tumbuh di dua lokasi yang berbeda ketinggian tempat yaitu di Kampus Fakultas Pertanian Unpatti dan Kampus IAIN Ambon. Sebanyak 10 isolat dan 7 isolat diperoleh dari daun dan batang Salawaku di kampus IAIN sedangkan 9 isolat yang terdiri atas 3 isolat dari dari akar, 2 isolat dari batang, dan 14 isolat dari daun Salawaku di Kampus Unpatti. Berdasarkan uji hipersensitif pada tembakau, 19 isolat tidak bersifat patogen. Pengecatan Gram memastikan bahwa 8 isolat sebagai Gram negatif dan 28 isolat sebagai Gram positif. Dari uji antagonis terhadap 18 isolat endofit asal Salawaku terhadap cendawan patogen Cercospora spp. diperoleh dua isolate bakter endofitik yang mampu menekan pertumbuhan patogen Cercospora EDMT14 dan EBBA1 dengan daya hambat berturut-turut 78,04% dan 76,12%.Kata Kunci: antagonis, bakteri endofit, Cercospora spp, Salawaku (Falcataria mollucana)
Endophytic bacteria is microbe lives in plant tissues without causing symptoms of disease. The benefit of utilizing endophytic bacteria as a technique for controlling diseases such as sprout (R. solani) has been known due to its ability to live and to colonize plant internal tissues. This study aims to determine the ability of endophytic bacteria from sago to suppress the growth of R. solani in vitro. This research was conducted using 19 isolates of endophytic bacteria from sago with 5 replications. The results showed that two endophytic isolates namely STA2 and STA13 were able to suppress the growth of R. solani pathogens compared to controls with inhibition percentage of 41.86% and 16.27%, respectively.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.