No abstract

The medicinal plants have gained significant importance in traditional medicine and pharmacy due to wide variety of curative properties. The medicinal properties of the plants rely on the presence of various phytoconstituents. The present study focused on screening of phytochemicals from different parts (leaf, stem, petiole, fruit and root) of Solanum khasianum using solvents with varying polarity like methanol, butanol, chloroform, acetonitrile and water. The qualitative screening of phytoconstituents showed the existence of alkaloids, flavonoids, phenols, tannins, steroids, saponins and absence of quinones in all 25 extracts tested. The quantitative determination showed that root extract contains considerable amounts of total flavonoids (214.74±0.45), total phenols (183.47±0.38), total alkaloids (132.46±0.28), total antioxidants (95.66±0.27), total tannins (14.66±0.07) whereas total saponins were present in high content in fruit methanolic extract (44.99±0.33). The presence of these phytoconstituents indicates the therapeutic potential of S. khasianum and scientifically validates the traditional use of this plant.

Objective: The immense literature study was carried out and disclosed that here no method arrived for the concomitant assessment of omeprazole and piperine in bulk form by using RP-HPLC. Hence, an effort was assembled to arise a easy, specific, precise, reliable, linear, rapid, and validated reverse phase-high-performance liquid chromatography (RP-HPLC) technique for the simultaneous assessment of omeprazole and piperine in bulk form.Methods: The chromatographic analysis of omeprazole and piperine was performed using a RP-HPLC (WATERS) provided with autosampler and ultraviolet (UV) detector with the software of EMPOWER Version 2. The chosen conditions were isocratic separation with two mobile phase composed of acetonitrile:buffer (phosphate buffer: pH 6.5 ± 0.1) (55:45). Detection was carried out using UV/visible double-beam spectrophotometer at 320 nm. The method was validated as per the ICH guidelines.Results: The retention time for omeprazole and piperine by proposed HPLC method was found to be 2.767 and 4.029 min, respectively. The correlation coefficients are 0.999. The developed chromatographic method was found to be accurate with recovery 99.2–99.8% and was found within the acceptance criteria (i.e., 98.0–102.0%) with acceptable % relative standard deviation of not >2% at each level.Conclusion: Thus, the proposed HPLC procedure for the concomitant assessment of omeprazole and piperine was accurate, precise, linear, robust, simple, and economic.