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Plants are excellent sources of secondary metabolites that have been used for the treatment of human diseases. The plant Momordica cymbalaria contains different types of phytochemicals like phenols, steroids, flavonoids, alkaloids and tannins. The present investigation was carried out to determine the bioactive compounds present in the different plant parts of Momordica cymbalaria by Gas chromatography-Mass spectroscopy (GC-MS) technique. Chromatograms and bioactive compounds of in vivo leaf, root and in vitro leaf callus methanolic extracts of Momordica cymbalaria are derived by GC-MS technique. 37 compounds were found in leaf methanolic extracts, 20 compounds in vitro leaf callus methanolic extracts and 18 compounds in root methanolic extracts. These are important bioactive compounds n-Hexadecanoic acid, 9-octadecenoic acid methyl ester,

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