Creating a good image: Macromolecular compounds comprising dextran, luminol, and biotin were used as nonenzymatic probes for the sensitive chemiluminescence imaging of a cytochrome P450 (CYP) protein on a poly(vinylidene difluoride) (PVDF) membrane (see picture). The polymeric framework is simply formed by mixing the probe with avidin in a ratio of 1:1. The target CYP3A4 protein can be detected rapidly at the femtomole level.
Auf das „Image“ kommt es an: Makromolekulare Verbindungen aus Dextran, Luminol und Biotin dienen als nichtenzymatische Sonden für die empfindliche Chemilumineszenz(CL)‐Bildgebung von Cytochrom P450 (CYP) an einer Poly(vinylidendifluorid)(PVDF)‐Membran (siehe Bild). Die polymere Gerüststruktur wurde einfach durch Mischen der Sonde mit Avidin im Verhältnis 1:1 erhalten. Das CYP3A4‐Protein wird schnell und in femtomolaren Mengen detektiert.
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