The research objective was to study local traditional wisdom of the art of producing fabric dye from fresh buffalo manure (FBM). Data were collected from rural weaving community cooperative (co-op) members and experts in Thailand’s northeastern Mekong River province of Nakhon Phanom. The study’s Method 1 added buffalo dung water, salt, and a mordant together, which was brought to a boil for 30 minutes. Method 2 added buffalo dung water and salt, which was brought to a boil for 10 minutes, after which a mordant agent was added, then boiled for an additional 30 minutes. There were five different formulas for each method using a different mordant. Results revealed that Method 1 had the best dyeing solution and produced a consistent, commercially usable, washable fabric with three-tone coloring and long-lasting yarn. Method 1 also used a hot dyeing technique in which cotton yarn and FBM were disinfected in a boiling vat while natural odor-reducing additives were added. Moreover, Method 1 consisted of either (1) FBM, salt, and lye, (2) FBM, salt, and muddy water, or (3) only salt added to FBM. These three solutions yielded a material in which testing by the Thailand Textile Institute (THTI) using ISO standards was determined to have a moderate degree of lightfastness, as well as an excellent resistance to color fading after washing. The study is important as it adds a wealth of knowledge to the literature concerning eco-friendly eco-fashion, sustainable products, and economically viable traditional handicraft textile production techniques.