Aim This study was aimed to investigate the role of TUG1 in LPS‐stimulated hPDLCs and to evaluate the potential functions of TUG1 in the pathogenesis of periodontitis. Methods LPS‐stimulated hPDLCs were established as the cell model. CCK‐8 assay was performed to assess cell proliferation ability. Flow cytometry was performed to detect cell cycle distribution, and quantitative RT‐PCR and Western blotting were conducted to measure gene expressions. ELISA kits were used to evaluate the production of inflammatory cytokines. The putative binding site between TUG1 and miR‐498 was verified using luciferase reporter and RNA immunoprecipitation assays. Results TUG1 was downregulated upon LPS stimulation in hPDLCs. TUG1 overexpression promoted cell proliferation through regulating the cell cycle distribution, along with the decreased expression of p21 and increased expression of CDK2 and cyclin D1. Besides, TUG1 overexpression decreased the production of inflammatory cytokines. The effects were opposite upon TUG1 knockdown. TUG1 negatively regulated its target miR‐498, and influenced the expression of RORA, the direct target of miR‐498. Simultaneous TUG1 overexpression and miR‐498 reversed the effect of TUG1 overexpression alone on alleviating LPS‐induced cell injury and inhibition of Wnt/β‐catenin signaling, which was further changeover after co‐overexpression with RORA. Conclusion Therefore, TUG1 could protect against periodontitis via regulating miR‐498/RORA mediated Wnt/β‐catenin signaling.

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Background: Periodontitis (PD) is a chronic inflammatory disease caused by infection of the periodontal supporting tissues. Clinical studies have reported that rheumatoid arthritis (RA) patients have a higher prevalence of PD. This study aimed to explore the correlation between RA and PD.Methods: A total of 307 RA patients (RA group) and 324 healthy individuals (control group) who received physical examinations during the same period were recruited to this study. The incidence of PD in the two groups was analyzed, and the periodontal disease index (PDI) and bleeding on probing (BOP) were recorded.Then, 42 RA patients with PD and 56 control group patients with PD were selected for further analysis.Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of interleukin 1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the gingival crevicular fluid (GCF) of the two groups. For patients with both RA and PD, the level of serum C-reactive protein (CRP) and the duration of morning stiffness were also recorded. Results:The prevalence of PD in the RA group (51.5%) was significantly higher than that in the control group (31.2%), and the prevalence of PD also increased notably with the increase of age and the duration of the disease in RA patients. The levels of TNF-α and IL-1β in the PDI and the GCF in the concurrent RA and PD group were significantly higher than those in the PD group (P<0.05). Partial correlation analysis showed that TNF-α in the GCF positively correlated with the BOP of patients with RA and PD. Multiple linear regression analysis showed that the level of TNF-α in the GCF and serum CRP were independent influencing factors of the level of IL-1β in the GCF (the r values were 1.074 and 3.851, respectively; P<0.01). Conclusions:The presence of RA can increase risk of PD occurrence and is positively correlated with the levels of IL-1β and TNF-α in the GCF.

Erectile dysfunction (ED) and chronic periodontal disease (CPD) share mutual risk factors, and the incidence of ED is increasing among young adults. The relation of CPD and ED remains obscure due to inconsistent clinical evidence. This study aimed to further assess the relationship between CPD and ED using the Community Periodontal Index of Treatment Need (CPITN) and the International Index of Erectile Function (IIEF). Totally, 202 adult men were included, with 100 subjects with ED in the case group and 102 subjects without ED undergoing routine dental examinations in the control group. The IIEF questionnaire was used to assess the severity of ED, and CPD was assessed through the Community Periodontal Index (CPI) score. Periodontal assessments were performed by one single calibrated examiner. Logistic regression analysis was performed for the association between CPD and ED. After adjustment for age, smoking status, tooth brushing time, education level, monthly income, tooth brushing frequency, and gum bleeding, higher CPI score was identified to be associated with a greater risk of ED (odds ratio [OR] = 2.755, 95% confidence interval [CI] = [1.400, 5.423], p = .003), suggesting that CPD was positively associated with the odds of ED. CPD was getting more severe with the progress of ED ( p < .05). Men with ED could be encouraged to receive routine dental examinations and appropriate preventive dental measures to maintain oral and periodontal health.

This clinical case report describes the fabrication of monolithic self-glazed zirconia prostheses via a digital workflow, for full-arch rehabilitation in a patient with a severely worn dentition. Prostheses composed of self-glazed zirconia are less prone to chipping, and have a lower risk of progressive wear of the opposing natural teeth. The use of a digital workflow not only ensures a higher degree of precision in the fabrication of self-glazed zirconia prostheses, but it also greatly improves patient comfort, and reduces the clinical chairside time required for adjustments.