HighlightsEtx mutant Y30A-Y196A showed markedly reduced cytotoxicity towards MDCK.2 cells.Y30A-Y196A is inactive in mice after intraperitoneal administration.Y30A-Y196A is able to induce a specific antibody response in rabbits.Y30A-Y196A polyclonal antibody is able to induce protective immunity in vitro.Y30A-Y196A could form the basis of a recombinant vaccine against enterotoxemia.
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