Abstract:The investigation was carried out to determine the possible chemical components and quantity of the component present in honey using GC-MS analysis. Traditionally the natural honey are used in the treatment of ulcers, wound healing, swells, asthma, cough, hyperacidity, leprosy, diuretic, antimicrobial, jaundice, diuretic activity, hypolipidemic effect, hepatoprotective activity and fever. In the present study, the honey has been subjected to GC-MS analysis. Fourteen chemical constituents have been identified, the major chemical constituents are 2, 4-Dimethyl-1-pentanol (9.23%), 3, 5-Dihydroxy-6-methyl-2, 3-dihydro-4H-pyran-4-one (8.91%), 2-Furancarboxaldehyde, 5-hydroxymethyl (36.02%), 2-Butoxyethyl acetate (11.11%). It could be concluded that the Ngaoundere, Cameroon honey contains various bioactive compounds.