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AIM: This study aimed to investigate Saurauia vulcani Korth. leaves. the activity of ethanol extract in hypoglycemic, superoxide dismutase (SOD), glycosylated haemoglobin (HbA1c) and detection of insulin expression by immunochemistry METHODS: Saurauia vulcani Korth. Leaves powder was extracted by maceration method with ethanol 96%. The extract was administrated orally in doses of 100 mg/kg BW for 27 days. Diabetes was induced in rats by administered of Nicotinamide (NA) 230 mg/kg BW and streptozotocin (STZ) 65 mg/kg BW. Level of blood glucose, SOD, HbA1c were measured, and histopathology pancreas was observed to determine insulin expression with immunochemistry RESULTS: Ethanol extract of Saurauia vulcani Korth. Leaves (EESL) shown a significantly (*p < 0.05) reduced in blood glucose levels at 104.25 ± 2.562 mg/dL and HbA1c level at 32.53 ± 0.188 ng/mL, but increased SOD level at 60.64 ± 0.740 pg/mL and histopathology study shown secretion insulin as seen number of expressions of insulin / slice 31.00 ± 0.315. CONCLUSION: The result of this study showed EESL possess the hypoglycemic activity and increase the level of SOD but decrease the level of HbA1c in diabetic rat condition. The mechanism of the activity is suggested by stimulating the insulin secretion of pancreas β-cells which were damaged.

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Tapak dara (Catharanthus roseus) is a plant belonging to the Apocynaceae family originating from Central America and is generally grown as an ornamental plant. The purpose of this study was to determine the percentage of mortality data and the LC50 value of tapak dara methanol extract (Catharanthus roseus) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method. The BSLT method used consisted of 6 concentration treatments, namely 20 ppm, 60 ppm, 100 ppm, 140 ppm, 180 ppm, and 0 ppm a negative control, each of which was repeated three times. At each concentration, 10 experimental animals of 48 hours old Artemia salina Leach larvae were used. The toxic effect of the extract was identified by the percentage of mortality of Artemia salina Leach larvae using probit analysis (LC50). The results showed the linear regression equation y = 3.0809x – 12.065. This shows that the mortality of the test animals reached 50% when the compound concentration reached 34,599 ppm. Based on the toxicity test of the methanol extract of the tapak dara flower (Catharanthus roseus) using the Brine Shrimp Lethality Test (BSLT) method, in this study it was toxic because LC50>1000 ppm so it has potential as an anticancer.

Herba Pugun Tanoh (Picria fel terrae L.), spesies tumbuhan dari famili Scrophulariceae. Penelitian ditujukan untuk penentuan persen kematian dan nilai LC50 dari ekstrak etanol herba Pugun Tanoh pada larva Artemia Salina L. secara Brine Shirmp Lethality Test (BSLT). Konsentrasi perlakuan meliputi 180,0 ppm; 200,0 ppm; 220,0 ppm; 240,0 ppm; 260,0 ppm; 280,0 ppm; serta 300,0 ppm serta 0,0 ppm sebagai kontrol negatif dengan perlakuan tiga kali ulangan. Setiap perlakuan digunakan larva berumur 48 jam sebanyak 10 ekor. Toksisitas diidentifikasi berdasar persen kematian larva dengan analisis probit (LC50). Hasil diperoleh persamaan linear, Y = 11,222X+65,427. Nilai LC50 tercapai saat konsentrasi 188,691 ppm. Toksisitas ekstrak etanol herba Pugun Tanoh diuji secara BSLT menunjukkan sifat toksik, ada potensi sebagai antikanker karena LC50 < 1000,0 ppm