Long non-coding RNAs (lncRNAs) play an important role in epigenetic regulation, and abnormalities may lead to male infertility. To investigate whether lncRNAs are involved in intergenerational inheritance of obesity and obesity-induced decline in fertility, we divided mice into obesity (F0 mice fed a high-fat diet, F0-HFD) and non-obese (F0 mice fed normal chow, F0-NC) model groups and their male offspring (F1-HFD and F1-NC, respectively). We examined the differences in the expression levels of lncRNAs and mRNAs in the F0-HFD/F0-NC and F1-HFD/F1-NC groups. The results revealed similar expression patterns in the F1-HFD/F0-HFD groups at both the lncRNA and mRNA levels. The maximum difference in the lncRNA expression was observed between the F0-HFD and F0-NC groups. The differentially expressed lncRNA targets and mRNAs identified in our study are mainly involved in GnRH signalling pathway, metabolic process, and Hippo signalling pathway; similarly expressed lncRNAs and mRNAs in F1-HFD/F0-HFD are closely linked with G-protein coupled receptor signalling pathway, pancreatic polypeptide receptor activity, and lysine biosynthesis, which may play an important role in the molecular mechanism of intergenerational inheritance of obesity. Furthermore, potential genes that might play important roles in the pathogenesis of obesity-related low fertility were revealed by lncRNA-and mRNA-interaction studies based on the microarray expression profiles. In conclusion, we found that lncRNA could be involved in obesity-induced infertility by expressing abnormalities, which could act as genetic vectors of paternal inheritance of obesity.

No abstract

No abstract

No abstract

A refractive index (RI) and temperature or a temperature and axial strain sensor based on an inline Mach–Zehnder interferometer with thin core fiber (TCF)–thin fiber (TF)–TCF structure is proposed and experimentally demonstrated, requiring only the cleaving and fusion splicing methods. The operation principle depends on the effect that the TF cladding modes interfere with the core mode as an optical coupler. The RI, temperature, or axial strain variations can lead to resonance dip variations in the interferometer spectra, and the RI, temperature, or axial strain sensitivity can be measured by monitoring the wavelength shifts of resonance dips. Then we can measure both RI and temperature, or temperature and axial strain through the demodulation matrix. Four sensors with different TF lengths are fabricated based on numerical simulation. A 15 mm long TF sensor displays an RI sensitivity as high as − 174.357 n m / R I U , temperature sensitivities in the glycerin solution and the air of 12.47 and 26.19 pm/°C, and axial strain sensitivity of − 3.43 × 10 − 4 n m / µ ε . Moreover, due to its simple manufacture, high cost-effectiveness and compactness, the proposed sensor has a broad application prospect in physical, chemical, and biological sensing.