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In this study we used spectrophotometrical techniques to estimate antioxidant efficacy by using 2,2-diphenyl-1-picrylhydrazyl free radical scavenging capacity and reducing power capacity. We have used also electrochemical techniques performed by cyclic voltammetry. The present study examined the antioxidant activities of the methanolic extract (ME) of date palm (Phoenix dactylifera. L) fruits (DPF) of four native cultivars from Algeria, namely, Ghars (Gh), Chtaya (Cht), Degla Beïda (DB) and Tinissine (Tns). Quantitative estimation of total phenol and flavonoid content by a colorimetric assay showed that methanol extracts are rich in these components. The content of total phenolics of different cultivars extracts was in the range of 26.083 - 35.850 GAE/100g DW. while it was the content of total flavonoids of the different cultivars extracts was in the range of 5.276 - 6.055 QE/100g DW. The value of IC50 for DPPH radical test ranged from 0.193 to 0.400mg/ml. All extracts showed very good activity of ferric reducing power, the higher power was in methanolic extract of Tns. The less IC50 value in cyclic voltammetry method (CV), which meet the highest effective antioxidant was 0.121 mg/ml in methanolic extract of Tns.

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The present investigation is aimed at screening the bioactive compounds present in the leaves of Pergularia tomentosa L. an important ethnomedicinal plant. The qualitative analysis for the present phytochemicals was performed using MeOH/H2O extracts. Phytochemical analysis showed the existence of some primary and secondary metabolites, phenolic, flavonoids, cardenolides, tannins, alkaloids, quinones, terpenoids and glycoside; leaves contained the highest amounts of all these compounds. The highest total phenolic content and total tannins was observed in aqueous extract which is 2.59±0.49 mg GAE/g DW and 0.436±0.00 mg CE/g DW respectively. In addition, the highest total flavonoid content was observed in butanol extract which is 0.486±0.01mg QE/g DW. Reducing power assay and DPPH were employed to measure antioxidant activity in vitro, extracts were the most powerful antioxidants for the two-assay in vitro system. In addition, we investigated in vitro anti-diabetic activity of different extracts. The dichloromethane extract of Pergularia tomentosa L. showed strong antidiabetic antidiabetic activity.