Reaction of alkyl, acetoxy, and silyl enol ethers of 3-(organosilyl)cyclohexanone with molecular dioxygen in toluene at 110 degrees C produced the corresponding conjugated enones in yields up to 88% yield. The reaction of the same type failed on replacement of the silyl group at the C-3 position with an isopropyl group. These results indicate the existence of an unprecedented silicon-induced ene-type reaction. Its reaction mechanism, generality, limitations, and exceptions are discussed.

We successfully determined a suitable glucose concentration for endothelial cells (ECs) using a gradientgenerating microfluidic chip and a micro-stamper that were fabricated using micro-electro-mechanical systems (MEMS) technology. Our strategy was to generate a stable concentration gradient in the observation area based on a microfluidic network and micro-mixers, which produced a concentration gradient under various flow rates. The areas for cell adhesion were delineated on a glass slide with a micro-stamper using the micro-contact printing (lCP) method. We also discuss which glucose concentration gradients are suitable for cell viability test (i.e., 0-0.2%, 0.05-0.15%, and 0.06-0.17%). After examining various concentration gradients, the suitable glucose concentration for EC's viability test was determined to range from 0.077% (4.2 mM) to 0.147% (8.16 mM). Higher or lower concentrations caused the ECs to atrophy or die. In this study, we describe a gradient-generating microfluidic chip that can be used to produce various drug concentrations for multi-concentration tests.

Organo-silicon compounds S 0060Silicon-Induced Ene-Type Reaction in the Thermal Conversion of Enolates to β-Silyl Enones with Molecular Dioxygen. -An appropriately chosen C-3-silyl group is crucial for the reaction. The method is applied to cyclohexanones and other types of substrates such as bridged (XI), polycyclic (XIII) and hindered (VII) enol acetates. -(HWU*, J. R.; CHEN, C. H.; HSU, C.-I.; DAS, A. R.; LI, Y. C.; LIN, L. C.;