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Single nucleotide polymorphisms (SNPs) are important biomarkers for evaluating sensitivity to drugs and for predicting whether people might have a disease in the future. In this study, we constructed an electrochemical detection system of an SNP of peroxisome proliferator-activated receptor γ 2 (PPARγ 2 ) (C34G) using glucose dehydrogenase (GDH) based on a single base extension method. Target DNA was hybridized with capture DNA immobilized on a gold electrode. Biotinylated dCTP was inserted to probe DNA if target DNA had the SNP. Avidin conjugated GDH can bind to one base extended DNA resulting in increased response current after washing of redundant avidin conjugated GDH and addition of glucose. In this system, PPARγ 2 (C34G) was detected specifically with 10 −8 mol dm −3 detection limit based on amperometric sensing system.