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In this study, fish samples were examined for the presence of extended spectrum β-lactamases (ESBL) producing Escherichia coli and Salmonella spp. Out of 200 samples collected from retail markets of Durg, Raipur, Rajnandgaon and Balod districts of Chhattisgarh State, 76 (38) and 46 (23%) samples were found contaminated with E. coli and Salmonella spp., respectively. Multiple antibiotic resistant (MAR) index varied between 0.00 to 0.88 and 0.11 to 0.88 for E. coli and Salmonella isolates, respectively. The isolates were screened phenotypically for ESBL production and multiplex PCR targeting bla TEM , bla SHV , andbla CTX-M genes, was carried out followed by sequencing. Prevalence of bla TEM , bla SHV and bla CTX-M genes in E. coli and Salmonella isolates were found to be 13.2, 1.3, 0 and 6.5, 0, 4.4%, respectively. Higher percentage of resistant genes was detected in plasmid DNA in comparison to genomic DNA. Sequencing of PCR amplicons of bla TEM and bla CTX-M genes revealed the presence of bla TEM-116 and bla CTX-M-15 variants, respectively. The study indicates the presence of multidrug resistant ESBL producing E. coli and Salmonella in fish samples, which may pose threat to the fish consumers.