The present study was conducted to evaluate and compare the in-vitro bioactive potential of various organic extracts of the root, stem and leaves of Urtica dioica and Urtica urens on growth of tested Gram Positive (+ve) and Gram negative (-ve) bacteria.The present investigation also includes the comparative chemical constituency of the crude extract of root, stem and leaves of both plants. The comparative bioactive potential of Urtica dioica and Urtica urens was tested against both pathogenic and non-pathogenic strains of bacteria that are Gram positive (Staphylococcus aureus and Bacillus subtilis) and Gram negative (Pseudomonas aeruginosa and E. Coli) conducted by using agar well diffusion method .The Minimum Inhibitory Concentrations (MICs)-the lowest concentration of antimicrobial agent that will inhibit any visible growth of microbein chloroform and methanol extracts of root, stem and leaves of both plants was determined using micro-well dilution method. For the phytochemical evaluation of plants metabolites-ascorbic acid, flavonoids, phenols and proteins has been quantitatively estimated. The MIC has been to be most effective in case of methanolic extract of root against both gram positive and gram negative bacteria. Significant antimicrobial activity has been observed against gram negative bacteria E. coli and both the gram positive bacteria testedexpressed by U. dioica comparatively more effective than U. urens. Only a moderate amount of activity is exhibited against P. aeruginosa by both the plants. The non-polar extracts were found to have comparatively higher bactericidal potential than the polar extracts. The phytochemicals analysis suggested the presence of ascorbic acid, phenols and proteins to be found in U. urens in significantly higher concentration than U. dioica. On the other hand, U. dioica has been found to be more abundant in flavonoid concentration comparatively.

The present study was conducted to evaluate the antioxidant and antimicrobial potential as well as the phytochemical composition of root, seed and leaves of Cichoriumintybus. The antioxidant activity was analyzed by detecting the 2, 2-Diphenyl-1- picrylhydrazyl  DPPH  radical  scavenging  potent  of  the constituents found  in  various  plant  parts  of Cichorium intybus,whereas for analyzing in-vitro antimicrobial  activity, agar well diffusion method has been opted. The antimicrobial  activity of plant was tested against both pathogenic as well as non-pathogenic species of bacteria by analyzing the corresponding inhibition zone exhibited by various  extracts  of  plant  parts  against  the  bacterial  growth.  Apart  from  this,  the  present  study  also  involves  the  quantitative phytochemical evaluation of total ascorbic acid in root, seed and leaves of Cichoriumintybus. The antioxidant potential of the plant was found to be comparatively lower in seed and leaves of plant than in root in accordance to the determined IC50 value . From the antimicrobial activity analysis, it was concluded that majority of times the non-polar extracts of all plant parts were found to be more effective  in inhibiting  growth  of bacteria.  Most significant  activity  has been  exhibited against  gram negative  bacteria  Pseudomonas aeruginosa. The ascorbic acid content in plant was found in the range of    2.253±0.867 to 12.594±2.05µg/ml. From the results of the conducted research, the importance  of plant as an antimicrobial and anti-oxidative  agent has been established. So, the plant can further be introduced as a potential drug for herbal medicine in the treatment of human disorders.