Renewed interest in studying auxin herbicides (WSSA group 4) is increasing due to the release of genetically engineered crop varieties that are tolerant to PRE and POST applications of specific formulations of dicamba. The development of auxin-resistant crops was in response to the development of herbicide-resistant weed species to glyphosate and other herbicides. Research was conducted at multiple field locations in Georgia in 2018 and 2019 to examine weed control when POST herbicides were applied to dicamba and glyphosate-resistant cotton at eight different points in time over a 24-hr period. Applications occurred at 1-hr prior to sunrise all the way up to midnight in one day to examine the effect of herbicide application timing on broadleaf weed control. Glyphosate, dicamba, and glyphosate plus dicamba were applied at each timing. Visual ratings of weed control were evaluated at 7, 14, 21, and 28 days after treatment (DAT). Weed control was affected by herbicide application timing. Midnight applications resulted in the lowest levels of control. Sicklepod, pitted morningglory, and prickly sida control was 49, 38, and 41% respectively. Greatest control of all three species (up to 99%) occurred from the noon to 1-hr prior to sunset application timings. Orthogonal contrasts of timing of application indicated that weed control was improved with day > night and pre-dawn > midnight.