New ostracod species are described for Middle to Upper Permian (Guadalupian) rocks of the Teresina Formation, Paraná Basin, from two localities in the São Paulo State, southwestern Brazil: Xavante Dam and Pau Preto Quarry. Paranacythere nigripallus gen. nov. and sp. nov., Velatomorpha xavante sp. nov., and Velatomorpha pseudoaltilis sp. nov. are herein described. The association Velatomorpha–Paranacythere is proposed as typical of Permian mixohaline environments of the Teresina Formation, and is the most abundant in the two studied localities. Paranacythere nigripallus gen. nov. and sp. nov. is proposed to accommodate a very abundant species with typically cytheroidean characteristics, and tentatively ascribed to the family Cytheruridae. Some ecological remarks are presented based on ostracod population age structure, biostratinomy and taxonomic composition of assemblages. The diagnosis of Velatomorpha Tibert & Dewey is emended. Keywords: evolution, marginal marine environments, paleoecology, Teresina Formation, taxonomy.