PSA level used as the main biomarker of prostate cancer is still controversial because it can provide a false positive result (Pentyala et al., 2016), while the use of a biopsy carries the risk of infectious complications (Wu et al., 2018), and can increase the risk of disease complications in patients with negative emotions (Wade et al., 2013).Therefore, supporting diagnostic methods are required to provide a more accurate diagnosis. A liquid biopsy such as urine can be a good option because it is noninvasive, simple, and can be repeated. In the body fluids, there are various cell and tissue products, including exosomes and extracellular nanovesicles which function as a medium of communication between cells. Exosomes enclose genetic