The aim of the present study was to assess the transcription of Listeria monocytogenes key virulence genes, namely sigB, prfA, hly, plcA, plcB, inlA, inlB, inlC and inlJ during subsistence on the surface of tomato, cucumber and carrot stored at 4, 10 and 30 °C for 0, 0.5, 6 and 24 h. Gene relative transcription was assessed through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results obtained, indicated that the relative transcription of plcA, plcB and inlB was more affected during subsistence on cucumber surface whereas the relative transcription of sigB, prfA, hly, inlA and inlC was more affected on tomato surface. Subsistence of the pathogen on carrot surface had only marginal effect on the relative transcription of the virulence genes assessed in the present study. In the majority of the cases, the aforementioned effects were dependent on the storage temperature employed.