These results indicate that CsA is directly toxic to LLC-PK1 cells with reduced DNA synthesis and cell cycle blockade. The mode of cell death, namely apoptosis or necrosis, is dose dependent. Fas may be an important mediator of CsA induced apoptosis in renal proximal tubular cells.

We studied the effects of cyclosporine A (CsA) on the proliferation of LLC-PK1 proximal tubule epithelial cells. DNA damage was found to be an early event in CsA nephrotoxicity and could be a sensitive indicator of CsA injury in renal epithelial cells. Cell cycle arrest induced by CsA was coincident with elevated p53 levels. It is possible that trans-activating p21 may mediate the halting of the cell cycle through the CsA-induced accumulation of p53.

Interaction between surfactants forms micelles that typically lead to a reduction of the irritation potential of the mixture. The objective of this study is to illustrate that such antagonism between surfactants is applicable to actual cases of consumer products e.g. laundry detergent products. The Zein test is an in vitro assay measuring corn protein denaturation by solutions of surfactant(s) and often used to predict their acute irritation potential. The Zein test data for the surfactant mixtures from nine representative laundry detergents demonstrate that the protein denaturation by the mixtures is, in all cases, lower than the cumulative score of protein denaturation by the different surfactants tested separately. The data clearly indicate that antagonism occurs between the actual surfactants present in their exact proportions as found in the consumer laundry detergent products. These data support the view that the hazard classification of detergent products as (irritant) by the dangerous preparation directive should not be based on a simple cumulative score of the irritation properties of the individual components of the products.

No abstract

No abstract